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Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology

Response surface methodology was used to determine optimum conditions for extraction of protein from fish waste using enzymatic method. The enzymatic method involved protease enzyme which are alcalase and protamex. Both methods investigated independent variables o C), time (60-240 such...

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Main Authors: Nurdiyana H., Siti Mazlina M. K., Ling T. C.
Format:
Published: 2013
Subjects:
Enzymatic method
degree of hydrolysis
Protein concentration
Online Access:http://ir.unikl.edu.my/jspui/handle/123456789/5235
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spelling my.unikl.ir-52352013-12-11T02:28:55Z Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology Nurdiyana H., Siti Mazlina M. K. Ling T. C. Enzymatic method degree of hydrolysis Protein concentration Response surface methodology was used to determine optimum conditions for extraction of protein from fish waste using enzymatic method. The enzymatic method involved protease enzyme which are alcalase and protamex. Both methods investigated independent variables o C), time (60-240 such as pH (7-11), temperature (40-60 minutes) and ratio of enzyme to sample (0.5-5). Selected response (dependent variable) which evaluates the extraction process for both methods were degree of hydrolysis (%) and protein concentration (mg/mL). From the results, extraction using enzymatic method-enzyme alcalase showed the highest yield of protein extracted (58.37%) and highest protein concentration (7.72 mg/ml) compared to enzymatic method-enzyme protamex. 2013-12-11T02:28:55Z 2013-12-11T02:28:55Z 2013-12-11 http://ir.unikl.edu.my/jspui/handle/123456789/5235
institution Universiti Kuala Lumpur
building UniKL Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kuala Lumpur
content_source UniKL Institutional Repository
url_provider http://ir.unikl.edu.my/
topic Enzymatic method
degree of hydrolysis
Protein concentration
spellingShingle Enzymatic method
degree of hydrolysis
Protein concentration
Nurdiyana H., Siti Mazlina M. K.
Ling T. C.
Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology
description Response surface methodology was used to determine optimum conditions for extraction of protein from fish waste using enzymatic method. The enzymatic method involved protease enzyme which are alcalase and protamex. Both methods investigated independent variables o C), time (60-240 such as pH (7-11), temperature (40-60 minutes) and ratio of enzyme to sample (0.5-5). Selected response (dependent variable) which evaluates the extraction process for both methods were degree of hydrolysis (%) and protein concentration (mg/mL). From the results, extraction using enzymatic method-enzyme alcalase showed the highest yield of protein extracted (58.37%) and highest protein concentration (7.72 mg/ml) compared to enzymatic method-enzyme protamex.
format
author Nurdiyana H., Siti Mazlina M. K.
Ling T. C.
author_facet Nurdiyana H., Siti Mazlina M. K.
Ling T. C.
author_sort Nurdiyana H., Siti Mazlina M. K.
title Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology
title_short Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology
title_full Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology
title_fullStr Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology
title_full_unstemmed Optimisation of Enzymatic Extraction of Fish Protein Using Response Surface Methodology
title_sort optimisation of enzymatic extraction of fish protein using response surface methodology
publishDate 2013
url http://ir.unikl.edu.my/jspui/handle/123456789/5235
_version_ 1644484699602550784
score 13.160551

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