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Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes

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Main Authors: Noorzaid Muhamad, LR Walker, DC Simcock, KC Pedley, HV Simpson, S Brown
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Published: Tehran University of Medical Sciences Publication 2013
Subjects:
Kinetics
Teladorsagia circumcincta
Urea
Online Access:http://ijpa.tums.ac.ir/index.php/ijpa/article/view/556/383
http://ir.unikl.edu.my/jspui/handle/123456789/2284
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spelling my.unikl.ir-22842013-05-30T09:29:10Z Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes Noorzaid Muhamad LR Walker DC Simcock KC Pedley HV Simpson S Brown Kinetics Teladorsagia circumcincta Urea Open access journal Background: Like several other parasites, Teladorsagia circumcincta secretes or excretes urea, but nei­ther the rate of efflux nor the possible metabolic sources of the urea has been considered. Methods: Parasites were maintained by passage through sheep. Urea efflux was measured using phe­nol/hypochlorite after treatment with urea aminohydrolase. The kinetics of creatine amidinohy­drolase and arginine amidinohydrolase were characterised by coupling the reactions with urea aminohy­drolase and glutamate dehydrogenase. Results: Infective L3 T. circumcincta secreted or excreted urea at 25% of the rate of NH3/NH­4+. The rate of urea efflux was about 84 pmol h‑1 (103 larvae)‑1 over 4 hours, corresponding to about 11 nmol h-1 mg-1 protein. We could not detect urea aminohydrolase activity, but urea production by both creatine amidinohydrolase and arginine amidinohydrolase could be detected. The apparent Km and Vmax of creatine amidinohydrolase were 1.1 mM and 48 nmol h-1 mg-1 protein, respectively, and the activity was greatest at pH 8. The apparent Km and Vmax of arginine amidinohydrolase were 0.7 mM and 62 nmol h-1 mg-1 protein, respectively, and the activity was greatest at pH 7.9. Conclusion: The activity of creatine amidinohydrolase and arginine amidinohydrolase was sufficient to account for the rate of urea secretion or excretion. 2013-05-30T09:29:10Z 2013-05-30T09:29:10Z 2013 Iranian Journal of Parasitology 2013. 8(1):137-144 http://ijpa.tums.ac.ir/index.php/ijpa/article/view/556/383 http://ir.unikl.edu.my/jspui/handle/123456789/2284 Iranian Journal of Parasitology; Tehran University of Medical Sciences Publication
institution Universiti Kuala Lumpur
building UniKL Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kuala Lumpur
content_source UniKL Institutional Repository
url_provider http://ir.unikl.edu.my/
topic Kinetics
Teladorsagia circumcincta
Urea
spellingShingle Kinetics
Teladorsagia circumcincta
Urea
Noorzaid Muhamad
LR Walker
DC Simcock
KC Pedley
HV Simpson
S Brown
Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
description Open access journal
format
author Noorzaid Muhamad
LR Walker
DC Simcock
KC Pedley
HV Simpson
S Brown
author_facet Noorzaid Muhamad
LR Walker
DC Simcock
KC Pedley
HV Simpson
S Brown
author_sort Noorzaid Muhamad
title Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
title_short Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
title_full Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
title_fullStr Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
title_full_unstemmed Urea Output by L3 Teladorsagia circumcincta and some Properties of Two Urea Producing Enzymes
title_sort urea output by l3 teladorsagia circumcincta and some properties of two urea producing enzymes
publisher Tehran University of Medical Sciences Publication
publishDate 2013
url http://ijpa.tums.ac.ir/index.php/ijpa/article/view/556/383
http://ir.unikl.edu.my/jspui/handle/123456789/2284
_version_ 1644484647927676928
score 13.214268

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