The role of hydrogen peroxide (H2O2) in sensing and signalling abiotic stimuli in rice

• Main Author: Nyuk, Ling Ma
• Format: Thesis
• Language: English
• Published: London : Imperial College London 2013
• Subjects: QD 502.5 .N9 2012; Nyuk, Ling Ma; Tesis Imperial College London 2012; Hydrogen peroxide; Organic reaction mechanisms
• Online Access: http://dspace.psnz.umt.edu.my/xmlui/handle/123456789/2722

This dissertation describes the integration of physiological, proteomic, and metabolomic changes in conjunction with the synthesis of H20 2 under various stress conditions in rice, Oryza sativa (Japonica var. Koshihikari). Dynamics of H202 production were observed in the leaves following the treatment with drought, salinity, osmotic and cold stress. The physiological parameters such as H202 content, photosynthetic activity, water content, and lipid peroxidation were used as indicators to correlate the physiological status of the plant with its metabolites and proteins profile/changes in response to stresses. A targeted proteomic approach has been developed to identify oxidative modified thiols, and a NMR-based analysis has been performed for metabolites profiling.