Effects of organic solvent and cationic additive on capillary electrophoresis of peptides
Capillary electrophoresis (CE) of nine peptides namely, bradykinin, bradykinin fragment 1-5, substance P, Arg8 -vasopressin, luteinizing hormone-releasing hormone (LHRH), bombesin, leucine-enkephalin, methionine-enkephalin and oxytocin were carried out using 0.5 % and 1.0 % formic acid (FA) as the s...
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my.ums.eprints.390602024-07-11T08:25:49Z https://eprints.ums.edu.my/id/eprint/39060/ Effects of organic solvent and cationic additive on capillary electrophoresis of peptides Noumie Surugau Ed T. Bergström David M. Goodall QD1-999 Chemistry QD71-142 Analytical chemistry Capillary electrophoresis (CE) of nine peptides namely, bradykinin, bradykinin fragment 1-5, substance P, Arg8 -vasopressin, luteinizing hormone-releasing hormone (LHRH), bombesin, leucine-enkephalin, methionine-enkephalin and oxytocin were carried out using 0.5 % and 1.0 % formic acid (FA) as the separation buffers, added with acetonitrile (ACN) and triethylamine (TEA) as an additive at low pH. The electrophoretic behaviour of these peptides was examined at different concentration of TEA (0, 10, 20, 30, 40 and 50 mM), and ACN (30, 40, 50, 60, 70 %) at their respective measured final pH. The results showed that all nine peptides were fully resolved with addition of 10 – 20 mM TEA. Peak efficiency was improved significantly by increasing TEA concentration up to 40 mM where 800 000 m-1 was obtained. Without TEA, the closely related enkephalins were co-migrating. Interestingly, by addition of as little as 5 mM TEA has sufficient to separate them almost at baseline. Increasing ACN to 40 % has shortened the analysis time by ca. 1 min. However, further increase of ACN can cause peak broadening and current instability. ResearchGate 2008 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/39060/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/39060/2/FULL%20TEXT.pdf Noumie Surugau and Ed T. Bergström and David M. Goodall (2008) Effects of organic solvent and cationic additive on capillary electrophoresis of peptides. The Malaysian Journal of Analytical Sciences, 12 (3). pp. 1-9. |
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QD1-999 Chemistry QD71-142 Analytical chemistry Noumie Surugau Ed T. Bergström David M. Goodall Effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
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Capillary electrophoresis (CE) of nine peptides namely, bradykinin, bradykinin fragment 1-5, substance P, Arg8 -vasopressin, luteinizing hormone-releasing hormone (LHRH), bombesin, leucine-enkephalin, methionine-enkephalin and oxytocin were carried out using 0.5 % and 1.0 % formic acid (FA) as the separation buffers, added with acetonitrile (ACN) and triethylamine (TEA) as an additive at low pH. The electrophoretic behaviour of these peptides was examined at different concentration of TEA (0, 10, 20, 30, 40 and 50 mM), and ACN (30, 40, 50, 60, 70 %) at their respective measured final pH. The results showed that all nine peptides were fully resolved with addition of 10 – 20 mM TEA. Peak efficiency was improved significantly by increasing TEA concentration up to 40 mM where 800 000 m-1 was obtained. Without TEA, the closely related enkephalins were co-migrating. Interestingly, by addition of as little as 5 mM TEA has sufficient to separate them almost at baseline. Increasing ACN to 40 % has shortened the analysis time by ca. 1 min. However, further increase of ACN can cause peak broadening and current instability. |
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Article |
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Noumie Surugau Ed T. Bergström David M. Goodall |
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Noumie Surugau Ed T. Bergström David M. Goodall |
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Noumie Surugau |
title |
Effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
title_short |
Effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
title_full |
Effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
title_fullStr |
Effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
title_full_unstemmed |
Effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
title_sort |
effects of organic solvent and cationic additive on capillary electrophoresis of peptides |
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ResearchGate |
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2008 |
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https://eprints.ums.edu.my/id/eprint/39060/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/39060/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/39060/ |
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