Preparation for de novo sequencing of ganoderma affecting oil palm in Sabah, Malaysia

• Format: Thesis
• Language: English; English
• Published: 2012
• Subjects: SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants
• Online Access: https://eprints.ums.edu.my/id/eprint/38636/1/ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/38636/2/FULLTEXT.pdf; https://eprints.ums.edu.my/id/eprint/38636/

Four types of preparation were done prior to de novo sequencing of Ganoderma sp. causing basal stem rot in oil palm located in Sabah, Malaysia. Firstly, at least 12 μg of DNA with the ratio of OD260 to OD2so between 1. 7 and 2.0 was obtained from the mycelia of Ganoerma sp. strain C in order to be s...