Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon
Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1)...
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Czech Academy of Agricultural Sciences
2024
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my.ums.eprints.381142024-02-02T07:48:34Z https://eprints.ums.edu.my/id/eprint/38114/ Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon Yawo Mawunyo Nevame Adedze Xia Lu Wenyi Fan Wenting Zhang Xue Yang Zhijun Deng Md. Amirul Alam Guangli Xu Lihua Zhang Wenhu Li CC73-81 Methodology QK900-989 Plant ecology Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F2 segregating population of melons generated from these two parents was inoculated by the PM pathogen. Based on bulk segregant analysis (BSA) using these 100 InDel markers, the powdery mildew resistance was associated with the genomic region LVpm12.1 on the melon chromosome 12. This region overlapped the previously described quantitative trait locus (QTL)-hotspot area carrying multiple PM-resistance QTLs. Moreover, conventional QTL mapping analysis was done, which located LVpm12.1 in the region between 22.72 and 23.34 Mb, where three highly polymorphic InDel markers MInDel89, MInDel92, and MInDel93 were detected. Therefore, these markers could be used to track this resistance locus in melon while the lines carrying this locus could be employed in PM melon resistance breeding programs after validation tests. Czech Academy of Agricultural Sciences 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38114/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38114/2/FULL%20TEXT.pdf Yawo Mawunyo Nevame Adedze and Xia Lu and Wenyi Fan and Wenting Zhang and Xue Yang and Zhijun Deng and Md. Amirul Alam and Guangli Xu and Lihua Zhang and Wenhu Li (2024) Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon. Czech Journal of Genetics and Plant Breeding, 60 (1). pp. 1-9. https://doi.org/10.17221/40/2023-CJGPB |
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CC73-81 Methodology QK900-989 Plant ecology Yawo Mawunyo Nevame Adedze Xia Lu Wenyi Fan Wenting Zhang Xue Yang Zhijun Deng Md. Amirul Alam Guangli Xu Lihua Zhang Wenhu Li Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon |
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Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F2 segregating population of melons generated from these two parents was inoculated by the PM pathogen. Based on bulk segregant analysis (BSA) using these 100 InDel markers, the powdery mildew resistance was associated with the genomic region LVpm12.1 on the melon chromosome 12. This region overlapped the previously described quantitative trait locus (QTL)-hotspot area carrying multiple PM-resistance QTLs. Moreover, conventional QTL mapping analysis was done, which located LVpm12.1 in the region between 22.72 and 23.34 Mb, where three highly polymorphic InDel markers MInDel89, MInDel92, and MInDel93 were detected. Therefore, these markers could be used to track this resistance locus in melon while the lines carrying this locus could be employed in PM melon resistance breeding programs after validation tests. |
format |
Article |
author |
Yawo Mawunyo Nevame Adedze Xia Lu Wenyi Fan Wenting Zhang Xue Yang Zhijun Deng Md. Amirul Alam Guangli Xu Lihua Zhang Wenhu Li |
author_facet |
Yawo Mawunyo Nevame Adedze Xia Lu Wenyi Fan Wenting Zhang Xue Yang Zhijun Deng Md. Amirul Alam Guangli Xu Lihua Zhang Wenhu Li |
author_sort |
Yawo Mawunyo Nevame Adedze |
title |
Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon |
title_short |
Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon |
title_full |
Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon |
title_fullStr |
Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon |
title_full_unstemmed |
Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis (BSA-seq) in melon |
title_sort |
development of pcr-based markers associated with powdery mildew resistance using bulked segregant analysis (bsa-seq) in melon |
publisher |
Czech Academy of Agricultural Sciences |
publishDate |
2024 |
url |
https://eprints.ums.edu.my/id/eprint/38114/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/38114/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/38114/ https://doi.org/10.17221/40/2023-CJGPB |
_version_ |
1789941997213057024 |
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13.160551 |