Effect of herbal plants on oxygen radical induced lipid peroxidation in liver homogenate

• Main Author: Chau Ann Lie
• Format: Academic Exercise
• Language: English; English
• Published: 2009
• Subjects: SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants
• Online Access: https://eprints.ums.edu.my/id/eprint/36421/1/24%20PAGES.pdf; https://eprints.ums.edu.my/id/eprint/36421/2/FULLTEXT.pdf; https://eprints.ums.edu.my/id/eprint/36421/

Several studies have shown the involvement of ROS in iron nitri)otriacetate (Fe-NTA)-induced liver toxicity, which may have relevance to its carcinogenicity. Fe-NTA is a potent nephrotoxic agent and induces acute and subacute renal proximal tubular necrosis and hepatic tumor promoter which are known to cause lipid peroxidation and DNA damage. Lipid peroxidation and DNA damage are the principal manifestation of Fe-NTA induced toxicity, which could be mitigated by antioxidants. Therefore, the aims of this study was to evaluate the effect of selected herbal plants viz. Orthosiphon stamineus., Lonicera japonica., Strobilanthes crispus., on oxygen radical-induced lipid peroxidation in rat liver homogenate for a possible protection against lipid peroxidation. The determination of lipid peroxidation was done by measuring the rate of production of thiobarbituric acid reacting substance (TBARS) expressed as malondialdehyde {MDA) equivalents and the results were expressed as n mol MDA formed/h/g tissue at 535 run using a spectrophotometer. Incubation of liver homogenate with hydrogen peroxide (40mM) in the presence of Fe-NTA (0.1 rnM) induces hepatic lipid peroxidation to about 2.3-fold as compared to saline treated control. The results demonstrate that at the highest dose of plant extract (50 ug/ml) used, higher inhibition was observed (P < 0.05). In lipid peroxidation protection studies, these plant extract treatment showed a dose-dependant inhibition (43%-57%, p < 0.05) of Fe-NTA and hydrogen peroxide - induced lipid peroxidation as measured by MDA. The decrease in the enhancement of lipid peroxidation depends on the dose used. It is concluded that these herbal plants may be a useful modulator of Fe-NTA and hydrogen peroxide-induced lipid peroxidation due its presence of polyphenolic compound might be responsible for such an effect.