Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats
Kelulut honey (KH) has been proven to have excellent antioxidative and anti-inflammatory properties with unique physicochemical characteristics. Therefore, we investigated the isolated and combined effects of KH, metformin, or clomiphene in alleviating oxidative stress and reproductive and metabolic...
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my.ums.eprints.350932023-02-13T04:08:01Z https://eprints.ums.edu.my/id/eprint/35093/ Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar QK710-899 Plant physiology R5-920 Medicine (General) Kelulut honey (KH) has been proven to have excellent antioxidative and anti-inflammatory properties with unique physicochemical characteristics. Therefore, we investigated the isolated and combined effects of KH, metformin, or clomiphene in alleviating oxidative stress and reproductive and metabolic abnormalities in polycystic ovary syndrome (PCOS). Female Sprague-Dawley (SD) rats were given 1 mg/kg/day of letrozole for 21 days to induce PCOS. PCOS rats were then divided into six treatment groups: untreated, metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were administered orally for 35 days. The physicochemical characteristics of KH were assessed through hydroxymethylfurfural, free acidity, diastase number, moisture content, sugar profile, metals, and mineral compounds. Additionally, we determined the semi volatile organic compounds present in KH through gas chromatography-mass spectrometry (GC/MS) analysis. KH and its combination with metformin or clomiphene were shown to improve the oestrus cycle, hormonal profile, and oxidative stress in PCOS rats. However, KH did not reduce the fasting blood glucose, insulin, and body weight gain in PCOS rats. These findings may provide a basis for future studies to discover the potential use of KH as a complementary treatment for women with PCOS MDPI 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/35093/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/35093/2/FULL%20TEXT.pdf Datu Agasi Mohd Kamal and Siti Fatimah Ibrahim and Azizah Ugusman and Mohd Helmy Mokhtar (2022) Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats. Antioxidants, 11. pp. 1-21. ISSN 2076-3921 https://www.mdpi.com/2076-3921/11/10/1879 https://doi.org/10.3390/antiox11101879 |
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QK710-899 Plant physiology R5-920 Medicine (General) Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats |
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Kelulut honey (KH) has been proven to have excellent antioxidative and anti-inflammatory properties with unique physicochemical characteristics. Therefore, we investigated the isolated and combined effects of KH, metformin, or clomiphene in alleviating oxidative stress and reproductive and metabolic abnormalities in polycystic ovary syndrome (PCOS). Female Sprague-Dawley (SD) rats were given 1 mg/kg/day of letrozole for 21 days to induce PCOS. PCOS rats were then divided into six treatment groups: untreated, metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were administered orally for 35 days. The physicochemical characteristics of KH were assessed through hydroxymethylfurfural, free acidity, diastase number, moisture content, sugar profile, metals, and mineral compounds. Additionally, we determined the semi volatile organic compounds present in KH through gas chromatography-mass spectrometry (GC/MS) analysis. KH and its combination with metformin or clomiphene were shown to improve the oestrus cycle, hormonal profile, and oxidative stress in PCOS rats. However, KH did not reduce the fasting blood glucose, insulin, and body weight gain in PCOS rats. These findings may provide a basis for future studies to discover the potential use of KH as a complementary treatment for women with PCOS |
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Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar |
author_facet |
Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar |
author_sort |
Datu Agasi Mohd Kamal |
title |
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats |
title_short |
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats |
title_full |
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats |
title_fullStr |
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats |
title_full_unstemmed |
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats |
title_sort |
kelulut honey ameliorates oestrus cycle, hormonal profiles, and oxidative stress in letrozole-induced polycystic ovary syndrome rats |
publisher |
MDPI |
publishDate |
2022 |
url |
https://eprints.ums.edu.my/id/eprint/35093/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/35093/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/35093/ https://www.mdpi.com/2076-3921/11/10/1879 https://doi.org/10.3390/antiox11101879 |
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