Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers
Bermudagrass is a widely distributed turf grasses use in Malaysia. Nevertheless, the genetic variation on molecular level of bermudagrass was insufficient. In this study, a sum of 15 cultivars were collected from different accessions in Malaysia and being analyzed using Inter-simple Sequence Repeat...
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Marsland Press / Zhengzhou University
2014
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my.ums.eprints.349212022-11-22T03:27:08Z https://eprints.ums.edu.my/id/eprint/34921/ Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers Yew, Swe Shyan Abdul Shukor Juraimi M.Y. Rafii M. Shabanimofrad Md. Amirul Alam Md. Kamal Uddin M. A. Hakim QK1-989 Botany SB1-1110 Plant culture Bermudagrass is a widely distributed turf grasses use in Malaysia. Nevertheless, the genetic variation on molecular level of bermudagrass was insufficient. In this study, a sum of 15 cultivars were collected from different accessions in Malaysia and being analyzed using Inter-simple Sequence Repeat Markers (ISSR). A total of 172 fragments were generated from 15 selected ISSR primers. There are 164 polymorphic bands with 95.3% of polymorphism. Fragment size ranged from 120 to 1867bp. The cultivars were clustered into 5 major groups at coefficient level of 0.5 through arithmetic average (UPGMA) cluster analysis of Jaccard’s similarity coefficient. The genetic similarity coefficient was range from 0.25 to 0.61 with an average of 0.43. Satiri and Melaka cultivars were clustered separately from their own hybrid and common clusters, respectively. These findings indicated that ISSR marker is an effective molecular method use to study genetic variation of bermudagrass and could be used for varietal development program. Marsland Press / Zhengzhou University 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34921/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34921/2/Full%20text.pdf Yew, Swe Shyan and Abdul Shukor Juraimi and M.Y. Rafii and M. Shabanimofrad and Md. Amirul Alam and Md. Kamal Uddin and M. A. Hakim (2014) Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers. Life Science Journal, 11. pp. 425-430. ISSN 1097-8135 (P-ISSN) , 2372-613X (E-ISSN) http://www.lifesciencesite.com/lsj/life1106/060_24209life110614_425_430.pdf |
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QK1-989 Botany SB1-1110 Plant culture Yew, Swe Shyan Abdul Shukor Juraimi M.Y. Rafii M. Shabanimofrad Md. Amirul Alam Md. Kamal Uddin M. A. Hakim Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers |
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Bermudagrass is a widely distributed turf grasses use in Malaysia. Nevertheless, the genetic variation on molecular level of bermudagrass was insufficient. In this study, a sum of 15 cultivars were collected from different accessions in Malaysia and being analyzed using Inter-simple Sequence Repeat Markers (ISSR). A total of 172 fragments were generated from 15 selected ISSR primers. There are 164 polymorphic bands with 95.3% of polymorphism. Fragment size ranged from 120 to 1867bp. The cultivars were clustered into 5 major groups at coefficient level of 0.5 through arithmetic average (UPGMA) cluster analysis of Jaccard’s similarity coefficient. The genetic similarity coefficient was range from 0.25 to 0.61 with an average of 0.43. Satiri and Melaka cultivars were clustered separately from their own hybrid and common clusters, respectively. These findings indicated that ISSR marker is an effective molecular method use to study genetic variation of bermudagrass and could be used for varietal development program. |
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Article |
author |
Yew, Swe Shyan Abdul Shukor Juraimi M.Y. Rafii M. Shabanimofrad Md. Amirul Alam Md. Kamal Uddin M. A. Hakim |
author_facet |
Yew, Swe Shyan Abdul Shukor Juraimi M.Y. Rafii M. Shabanimofrad Md. Amirul Alam Md. Kamal Uddin M. A. Hakim |
author_sort |
Yew, Swe Shyan |
title |
Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers |
title_short |
Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers |
title_full |
Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers |
title_fullStr |
Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers |
title_full_unstemmed |
Genetic Divergence of Bermudagrass (Cynodon spp.) Population Using ISSR Markers |
title_sort |
genetic divergence of bermudagrass (cynodon spp.) population using issr markers |
publisher |
Marsland Press / Zhengzhou University |
publishDate |
2014 |
url |
https://eprints.ums.edu.my/id/eprint/34921/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34921/2/Full%20text.pdf https://eprints.ums.edu.my/id/eprint/34921/ http://www.lifesciencesite.com/lsj/life1106/060_24209life110614_425_430.pdf |
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13.15806 |