Root distribution in intercropping systems – a comparison of DNA based methods and visual distinction of roots

• Main Authors: Affendy Hassan, Dorte Bodin Dresbølla, Camilla Ruø Rasmussen, Annemette Lyhne-Kjærbye, Mette Haubjerg Nicolaisen, Michaela Schiller Stokholm, Ole Søgaard Lund, Kristian Thorup-Kristensen
• Format: Article
• Language: English; English
• Published: Informa UK Limited 2019
• Subjects: QK640-(707) Plant anatomy
• Online Access: https://eprints.ums.edu.my/id/eprint/34021/1/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots.pdf; https://eprints.ums.edu.my/id/eprint/34021/2/Root%20distribution%20in%20intercropping%20systems%20%E2%80%93%20a%20comparison%20of%20DNA%20based%20methods%20and%20visual%20distinction%20of%20roots1.pdf; https://eprints.ums.edu.my/id/eprint/34021/; https://www.tandfonline.com/doi/full/10.1080/03650340.2019.1675872; https://doi.org/10.1080/03650340.2019.1675872

This study was performed to i) compare root distribution of legume/nonlegume intercrops determined by qPCR and amplicon sequencing (Metabarcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying roo...