Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution

• Main Authors: Suthinee Sangkanu, Watcharapong Mitsuwan, Tooba Mahboob, Wilawan Mahabusarakam, Sopa Chewchanwuttiwong, Panisara Siphakdi, Tajudeen O. Jimoh, Polrat Wilairatana, Karma G. Dolma, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Patrice Andie Wiart, Roghayeh Norouzi, Abolghasem Siyadatpanah, Polydor Ngoy Mutombo, Veeranoot Nissapatorn
• Format: Article
• Language: English; English
• Published: Elsevier B.V. 2021
• Subjects: QL360-599.82 Invertebrates
• Online Access: https://eprints.ums.edu.my/id/eprint/33223/1/Phytochemical%2C%20anti-Acanthamoeba%2C%20and%20anti-adhesion%20properties%20of%20Garcinia%20mangostana%20flower%20as%20preventive%20contact%20lens%20solution%20.pdf; https://eprints.ums.edu.my/id/eprint/33223/2/Phytochemical%2C%20anti-Acanthamoeba%2C%20and%20anti-adhesion%20properties%20of%20Garcinia%20mangostana%20flower%20as%20preventive%20contact%20lens%20solution%201.pdf; https://eprints.ums.edu.my/id/eprint/33223/; https://www.sciencedirect.com/science/article/pii/S0001706X21004447?casa_token=j_udSWBJMOAAAAAA:MpbXmiJTAnjslKKhjZKEZ5HpQCiyMcWexrClG2g7sQCZKzPYN-J5U63i7nh73JCYfBmRsptiSQ; https://doi.org/10.1016/j.actatropica.2021.106266

Acanthamoeba keratitis infection extends due to the growing number of contact lens users. Indigenous plants including Garcinia mangostana play a vital role in human health and well being. Many species of this plant have been reported with myriads of potent medicinal properties. However, the aims of this study were, for the first time, to isolate compounds from the flower of G. mangostana and to test their anti-Acanthamoeba and antiadhesion activity against Acanthamoeba triangularis. Powdered flowers of G. mangostana were extracted and chromatographed on a silica gel column. The structures of the compounds were established with the aid of 1 H NMR. More so, the anti-Acanthamoeba and anti-adhesion properties were tested on a 96-well polystyrene microtiter plate and soft contact lenses. Scanning electron microscope (SEM) was used to determine the features of A. triangularis on contact lenses. Eight pure compounds were obtained, namely 9-hydroxycalabaxanthone, tovophillin A, garcinone E, garcinone B, α-mangostin, gartinin, 8-deoxygartinin and γ-mangostin. The extract and pure compounds exhibited anti-Acanthamoeba activity with MIC values in the range of 0.25–1 mg/mL. In addition, the extract and α-mangostin displayed significant activity against the adhesion of A. triangularis trophozoites both in polystyrene plate and in contact lenses at 0.5 × MIC (0.25 mg/mL). Furthermore, α-mangostin has the potential to remove A. triangularis adhesion in contact lenses similar to a commercial multipurpose solution (MPS). SEM study confirmed that crude extract and α-mangostin are effective as solutions for contact lenses, which removed A. triangularis trophozoites within 24 h. Alpha-mangostin was non-toxic to Vero cells at a concentration below 39 μM in 24 h. Crude extract of G. mangostana flower and its α-mangostin serve as candidate compounds in the treatment of Acanthamoeba infection or as lens care solution, since they can be used as a source of natural products against Acanthamoeba and virulence factor associated with the adhesion of A. triangularis.