Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
The continuous discharge of toxic materials into the environment has been an alarming issue faces around the globe. Hence, matching effort of monitoring activity is vital to coping with the overwhelming amount of metal ions. Along with the significant current research being conducted, this study aim...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Tamkang University Press
2021
|
Subjects: | |
Online Access: | https://eprints.ums.edu.my/id/eprint/31965/2/Acetylcholinesterase%20%28AChE%29%20of%20Diodon%20hystrix%20brain%20as%20an%20alternative%20biomolecule%20in%20heavy%20metals%20biosensing_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31965/1/Acetylcholinesterase%20%28AChE%29%20of%20Diodon%20hystrix%20brain%20as%20an%20alternative%20biomolecule%20in%20heavy%20metals%20biosensing.pdf https://eprints.ums.edu.my/id/eprint/31965/ http://jase.tku.edu.tw/articles/jase-202206-25-3-0014.pdf http://dx.doi.org/10.6180/jase.202206_25(3).0014 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.ums.eprints.31965 |
---|---|
record_format |
eprints |
spelling |
my.ums.eprints.319652022-03-18T09:11:23Z https://eprints.ums.edu.my/id/eprint/31965/ Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah QH540-549.5 Ecology The continuous discharge of toxic materials into the environment has been an alarming issue faces around the globe. Hence, matching effort of monitoring activity is vital to coping with the overwhelming amount of metal ions. Along with the significant current research being conducted, this study aims to investigate the sensitivity of acetylcholinesterase (AChE) of Sabah porcupine fish, Diodon hystrix as an alternative biosensor in the detection of heavy metals. The enzyme was precipitated followed by the purification using ammonium sulfate precipitation and procainamide-affinity chromatography, respectively, with a total recovery of 66.67% with the specific activity of 2297.50 U/mg. The enzyme works optimally at pH 9 with the best incubation temperature of 30°C. The Michaelis constant (Km) and maximal velocity (Vmax) of 1.171 mM and 879257 mol/min/mg denotes the highest catalytic efficiency (Vmax/Km) of acetylthiocholine iodide (ATC) as its preferable substrate. Inhibition study tested on 10 metal ions resulted in increasing toxicity order of Cr6+ < Co2+ < Ag2+ < Cu2+ < Pb2+ < As5+ < Cd2+ < Zn2+ < Ni2+ < Hg2+, with only Hg2+ exhibited the half-maximal inhibitory concentration (IC50) of 0.48 mg/L. From the study, it suggests that the D. hystrix AChE as the potential conventional biosensor for heavy metals detection. Tamkang University Press 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31965/2/Acetylcholinesterase%20%28AChE%29%20of%20Diodon%20hystrix%20brain%20as%20an%20alternative%20biomolecule%20in%20heavy%20metals%20biosensing_ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/31965/1/Acetylcholinesterase%20%28AChE%29%20of%20Diodon%20hystrix%20brain%20as%20an%20alternative%20biomolecule%20in%20heavy%20metals%20biosensing.pdf Noreen Nordin and Rahmath Abdulla and Siti Aqlima Ahmad and Mohd Khalizan Sabullah (2021) Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing. Journal of Applied Science and Engineering, 25. pp. 473-480. ISSN 1560-6686 http://jase.tku.edu.tw/articles/jase-202206-25-3-0014.pdf http://dx.doi.org/10.6180/jase.202206_25(3).0014 |
institution |
Universiti Malaysia Sabah |
building |
UMS Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sabah |
content_source |
UMS Institutional Repository |
url_provider |
http://eprints.ums.edu.my/ |
language |
English English |
topic |
QH540-549.5 Ecology |
spellingShingle |
QH540-549.5 Ecology Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
description |
The continuous discharge of toxic materials into the environment has been an alarming issue faces around the globe. Hence, matching effort of monitoring activity is vital to coping with the overwhelming amount of metal ions. Along with the significant current research being conducted, this study aims to investigate the sensitivity of acetylcholinesterase (AChE) of Sabah porcupine fish, Diodon hystrix as an alternative biosensor in the detection of heavy metals. The enzyme was precipitated followed by the purification using ammonium sulfate precipitation and procainamide-affinity chromatography, respectively, with a total recovery of 66.67% with the specific activity of 2297.50 U/mg. The enzyme works optimally at pH 9 with the best incubation temperature of 30°C. The Michaelis constant (Km) and maximal velocity (Vmax) of 1.171 mM and 879257 mol/min/mg denotes the highest catalytic efficiency (Vmax/Km) of acetylthiocholine iodide (ATC) as its preferable substrate. Inhibition study tested on 10 metal ions resulted in increasing toxicity order of Cr6+ < Co2+ < Ag2+ < Cu2+ < Pb2+ < As5+ < Cd2+ < Zn2+ < Ni2+ < Hg2+, with only Hg2+ exhibited the half-maximal inhibitory concentration (IC50) of 0.48 mg/L. From the study, it suggests that the D. hystrix AChE as the potential conventional biosensor for heavy metals detection. |
format |
Article |
author |
Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah |
author_facet |
Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah |
author_sort |
Noreen Nordin |
title |
Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
title_short |
Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
title_full |
Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
title_fullStr |
Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
title_full_unstemmed |
Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
title_sort |
acetylcholinesterase (ache) of diodon hystrix brain as an alternative biomolecule in heavy metals biosensing |
publisher |
Tamkang University Press |
publishDate |
2021 |
url |
https://eprints.ums.edu.my/id/eprint/31965/2/Acetylcholinesterase%20%28AChE%29%20of%20Diodon%20hystrix%20brain%20as%20an%20alternative%20biomolecule%20in%20heavy%20metals%20biosensing_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31965/1/Acetylcholinesterase%20%28AChE%29%20of%20Diodon%20hystrix%20brain%20as%20an%20alternative%20biomolecule%20in%20heavy%20metals%20biosensing.pdf https://eprints.ums.edu.my/id/eprint/31965/ http://jase.tku.edu.tw/articles/jase-202206-25-3-0014.pdf http://dx.doi.org/10.6180/jase.202206_25(3).0014 |
_version_ |
1760230962454593536 |
score |
13.160551 |