Soluble expression and catalytic properties of codon-optimized recombinant bromelain from MD2 pineapple in Escherichia coli

• Main Authors: Rafida Razali, Cahyo Budiman, Khairul Azfar Kamaruzaman, Vijay Kumar Subbiah
• Format: Article
• Language: English; English
• Published: Springer 2021
• Subjects: QR1-502 Microbiology
• Online Access: https://eprints.ums.edu.my/id/eprint/30896/1/Soluble%20expression%20and%20catalytic%20properties%20of%20codon-optimized%20recombinant%20bromelain%20from%20MD2%20pineapple%20in%20Escherichia%20coli_ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/30896/2/Soluble%20expression%20and%20catalytic%20properties%20of%20codon-optimized%20recombinant%20bromelain%20from%20MD2%20pineapple%20in%20Escherichia%20coli.pdf; https://eprints.ums.edu.my/id/eprint/30896/; https://link.springer.com/content/pdf/10.1007/s10930-021-09974-9.pdf; https://doi.org/10.1007/s10930-021-09974-9

Bromelain, a member of cysteine proteases, is found abundantly in pineapple (Ananas comosus), and it has a myriad of versatile applications. However, attempts to produce recombinant bromelain for commercialization purposes are challenging due to its expressibility and solubility. This study aims to...