Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents
A simple, fast, repeatable, and less laborious sample-preparation protocol based on gas chromatography–mass spectrometry was developed and applied for the analysis of bioactive compounds derived from the filamentous fungus Aspergillus niger strain SS10. The match factors for the spectra of the sampl...
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my.ums.eprints.306952021-10-26T04:58:44Z https://eprints.ums.edu.my/id/eprint/30695/ Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents Shafiquzzaman Siddiquee Sujjat Al Azad Fatimah Abu Bakar Laila Naher Vijay Kumar Subbiah QA299.6-433 Analysis QR1-502 Microbiology A simple, fast, repeatable, and less laborious sample-preparation protocol based on gas chromatography–mass spectrometry was developed and applied for the analysis of bioactive compounds derived from the filamentous fungus Aspergillus niger strain SS10. The match factors for the spectra of the samples with reference to the mass-spectral library of fungal volatile compounds were determined and used to study the complex hydrocarbons and other volatile compounds that were separated using two different capillary columns and nonpolar and medium-polar stationary phases. More than 295 volatile compounds (spectral match factor of at least 90%), such as normal saturated hydrocarbons (C7–C30), cyclohexane, cyclopentane, fatty acids, alcohols, esters, sulfur- and bromo-containing compounds, simple pyrane, and benzene derivatives, were identified. Most of these compounds have not been reported earlier. The method described in this article is a suitable research tool for the determination of volatile compounds from the cultures of A. niger. Elsevier B.V. 2012-03-07 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30695/1/Separation%20and%20identification%20of%20hydrocarbons%20and%20other%20volatile%20compounds%20from%20cultures%20of%20aspergillus%20-Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/30695/2/Separation%20and%20identification%20of%20hydrocarbons%20and%20other%20volatile%20compounds%20from%20cultures%20of%20Aspergillus%20niger%20by%20GC-MS%20using%20two%20different%20capillary%20columns%20and%20solvents.pdf Shafiquzzaman Siddiquee and Sujjat Al Azad and Fatimah Abu Bakar and Laila Naher and Vijay Kumar Subbiah (2012) Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents. Journal of Saudi Chemical Society, 19. pp. 243-256. ISSN 1319-6103 https://www.researchgate.net/publication/https://www.sciencedirect.com/science/article/pii/S1319610312000373?via%3Dihub224928476_Separation_and_identification_of_hydrocarbons_and_other_volatile_compounds_from_cultures_of_Aspergillus_niger_by_GC-MS_using_two_different_capillary_columns_and_solvents http://doi.org/10.1016/j.jscs.2012.02.007 |
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QA299.6-433 Analysis QR1-502 Microbiology Shafiquzzaman Siddiquee Sujjat Al Azad Fatimah Abu Bakar Laila Naher Vijay Kumar Subbiah Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents |
description |
A simple, fast, repeatable, and less laborious sample-preparation protocol based on gas chromatography–mass spectrometry was developed and applied for the analysis of bioactive compounds derived from the filamentous fungus Aspergillus niger strain SS10. The match factors for the spectra of the samples with reference to the mass-spectral library of fungal volatile compounds were determined and used to study the complex hydrocarbons and other volatile compounds that were separated using two different capillary columns and nonpolar and medium-polar stationary phases. More than 295 volatile compounds (spectral match factor of at least 90%), such as normal saturated hydrocarbons (C7–C30), cyclohexane, cyclopentane, fatty acids, alcohols, esters, sulfur- and bromo-containing compounds, simple pyrane, and benzene derivatives, were identified. Most of these compounds have not been reported earlier. The method described in this article is a suitable research tool for the determination of volatile compounds from the cultures of A. niger. |
format |
Article |
author |
Shafiquzzaman Siddiquee Sujjat Al Azad Fatimah Abu Bakar Laila Naher Vijay Kumar Subbiah |
author_facet |
Shafiquzzaman Siddiquee Sujjat Al Azad Fatimah Abu Bakar Laila Naher Vijay Kumar Subbiah |
author_sort |
Shafiquzzaman Siddiquee |
title |
Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents |
title_short |
Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents |
title_full |
Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents |
title_fullStr |
Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents |
title_full_unstemmed |
Separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by GC-MS using two different capillary columns and solvents |
title_sort |
separation and identification of hydrocarbons and other volatile compounds from cultures of aspergillus niger by gc-ms using two different capillary columns and solvents |
publisher |
Elsevier B.V. |
publishDate |
2012 |
url |
https://eprints.ums.edu.my/id/eprint/30695/1/Separation%20and%20identification%20of%20hydrocarbons%20and%20other%20volatile%20compounds%20from%20cultures%20of%20aspergillus%20-Abstract.pdf https://eprints.ums.edu.my/id/eprint/30695/2/Separation%20and%20identification%20of%20hydrocarbons%20and%20other%20volatile%20compounds%20from%20cultures%20of%20Aspergillus%20niger%20by%20GC-MS%20using%20two%20different%20capillary%20columns%20and%20solvents.pdf https://eprints.ums.edu.my/id/eprint/30695/ https://www.researchgate.net/publication/https://www.sciencedirect.com/science/article/pii/S1319610312000373?via%3Dihub224928476_Separation_and_identification_of_hydrocarbons_and_other_volatile_compounds_from_cultures_of_Aspergillus_niger_by_GC-MS_using_two_different_capillary_columns_and_solvents http://doi.org/10.1016/j.jscs.2012.02.007 |
_version_ |
1760230797989642240 |
score |
13.18916 |