Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells

• Main Authors: Perng Yang Puah, Umul Hanim Yusof, Lee, Ping Chin, Moh, Pak Yan, How, Siew Eng
• Format: Article
• Language: English; English
• Published: Taylor & Francis Group 2020
• Subjects: Q Science (General); QM Human anatomy
• Online Access: https://eprints.ums.edu.my/id/eprint/30157/1/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells.pdf; https://eprints.ums.edu.my/id/eprint/30157/2/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells1.pdf; https://eprints.ums.edu.my/id/eprint/30157/; https://medpubresearch.com/wp-content/uploads/2020/03/Surface-characterization-biocompatibility-and-osteogenic-differentiation-of-drop-casted-multilayer-graphene-oxide-film-towards-human-whartons-jelly-derived-mesenchymal-stem-cells.pdf; https://doi.org/10.1080/10667857.2019.1674506

Graphene oxide (GO) materials have been extensively employed in mesenchymal stem cell (MSCs) research due to its unique nano topography. Herein, various concentrations of GO flakes were used to fabricate different thickness of multilayer graphene oxide (m-GO) films using a simple drop casting method and characterized by FTIR and AFM. The biocompatibility of m-GO films in culturing WJ-MSCs was investigated based on cell morphology, cell viability and osteogenic differentiation ability. Importantly, WJ-MSCs adhered and proliferated successfully on the m-GO films (6.25 µg, 12.5 µg and 25 µg) and showed no difference in cell morphology and viability after 5 days culture. Moreover, the WJ-MSCs growth on GO films (6.25 µg, 12.5 µg and 25 µg) enhanced the osteogenic differentiation as compared to the control (glass coverslip). Hence, the simple and inexpensive drop-casted fabrication strategy could provide biocompatible m-GO films to unlock the wider potential of WJ-MCSs in tissue engineering application.