The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions

• Main Authors: Mohd Khalizan Sabullah, Mohd Rosni Sulaiman, Mohd Yunus Abd Shukor, Mohd Arif Syed, Nor Aripin Shamaan, Ariff Khalid, Siti Aqlima Ahmad
• Format: Article
• Language: English; English
• Published: Hindawi Publishing Corporation 2014
• Subjects: QD Chemistry; SH Aquaculture. Fisheries. Angling
• Online Access: https://eprints.ums.edu.my/id/eprint/27463/1/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/27463/2/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20FULL%20TEXT.pdf; https://eprints.ums.edu.my/id/eprint/27463/; https://downloads.hindawi.com/journals/tswj/2014/571094.pdf

Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 μmole/min/mg and 0.23 mM...