Multi-step ft-ir spectroscopy analysis of the metabolite content in hibiscus sabdariffa var. UKMR-2 in response to soil media

• Main Authors: Siti Aishah Mohd Ali, J. Latip
• Format: Proceedings
• Language: English; English
• Published: Faculty of Science and Natural Resources 2020
• Subjects: Q Science (General); QK Botany
• Online Access: https://eprints.ums.edu.my/id/eprint/26960/1/The%20influence%20of%20modified%20equipment%20in%20developing%20skills%20in%20badminton.pdf; https://eprints.ums.edu.my/id/eprint/26960/2/The%20influence%20of%20modified%20equipment%20in%20developing%20skills%20in%20badminton1.pdf; https://eprints.ums.edu.my/id/eprint/26960/; https://www.ums.edu.my/fssa/wp-content/uploads/2020/12/PROCEEDINGS-BOOK-ST-2020-e-ISSN.pdf

The present study analyze the differences in metabolite content in UKMR-2 calyces in response to different soil media formulation ratio treatment by applying multi-step infrared based fingerprinting. The cultivation using two different media ratio (top soil, organic matter and sand); SM1 with 2:1:1 v/v/v and SM2 with 2:1:2 v/v/v. Multi-step infrared based fingerprinting: one dimensional infrared (1D-IR), second derivative infrared (SD-IR) and two dimensional correlation infrared (2D-IR) spectroscopy were used. The 1D-IR spectrums revealed a similar absorption in the fingerprint zone (1900 - 650 cm-1). SD-IR spectrums clearly showed have more peaks with different shape, position and intensity in the fingerprint zone. 2D-IR synchronous correlation spectrum clearly distinguished the metabolite content in the UKMR-2 calyx extract in the range of 1000 – 650 cm-1 . Therefore, multi-step infrared based fingerprinting has the potential as one of the effective methods to discriminate extract samples with similar infrared fingerprint features.