Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells

• Main Authors: Mohamed Qasim Al-Fahdawi, Faris A.J. Al-Doghachi, Qasim Khlaif Abdullah, Ruaa Tareq Hammad, Abdullah Rasedee, Wisam Nabeel Ibrahim, Hussah Abdullah Alshwyeh, Areej A Alosaimi, Sahar Khamees Aldosary, Eltayeb E. M. Eid, Rozita Rosli, Y.H. Taufiq-Yap, Nagi A. Al-Haj, Mothanna Sadiq Al-Qubaisi
• Format: Article
• Language: English; English
• Published: Elsevier Masson SAS 2021
• Subjects: R Medicine (General)
• Online Access: https://eprints.ums.edu.my/id/eprint/26750/3/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf; https://eprints.ums.edu.my/id/eprint/26750/2/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf; https://eprints.ums.edu.my/id/eprint/26750/; https://doi.org/10.1016/j.biopha.2021.111483

The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinumdoped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive Xray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/ MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anticancer compound.