Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides
Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun...
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my.ums.eprints.249842021-04-08T14:49:08Z https://eprints.ums.edu.my/id/eprint/24984/ Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides Mailin Misson Bo Jin Sheng Dai Hu Zhang Q Science (General) Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at are sidence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis. 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/24984/7/Interfacial%20Biocatalytic%20Performance%20of%20Nanofiber-Supported%20%CE%B2-Galactosidase%20for%20Production%20of%20Galacto-Oligosaccharides.pdf Mailin Misson and Bo Jin and Sheng Dai and Hu Zhang (2020) Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides. Catalysts, 10 (81). pp. 1-12. https://doi.org/doi:10.3390/catal10010081 |
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Q Science (General) Mailin Misson Bo Jin Sheng Dai Hu Zhang Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides |
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Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at are sidence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis. |
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Article |
author |
Mailin Misson Bo Jin Sheng Dai Hu Zhang |
author_facet |
Mailin Misson Bo Jin Sheng Dai Hu Zhang |
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Mailin Misson |
title |
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides |
title_short |
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides |
title_full |
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides |
title_fullStr |
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides |
title_full_unstemmed |
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides |
title_sort |
interfacial biocatalytic performance of nanofiber-supported β-galactosidase for production of galacto-oligosaccharides |
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2020 |
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https://eprints.ums.edu.my/id/eprint/24984/7/Interfacial%20Biocatalytic%20Performance%20of%20Nanofiber-Supported%20%CE%B2-Galactosidase%20for%20Production%20of%20Galacto-Oligosaccharides.pdf https://eprints.ums.edu.my/id/eprint/24984/ https://doi.org/doi:10.3390/catal10010081 |
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