Genome sequencing of three Vibriophages capable of controlling Pathogenic Vibrio bacteria in aquaculture

• Main Author: Julian Ransangan
• Format: Research Report
• Language: English
• Published: Universiti Malaysia Sabah 2015
• Subjects: SH Aquaculture. Fisheries. Angling
• Online Access: https://eprints.ums.edu.my/id/eprint/24583/1/Genome%20sequencing%20of%20three%20Vibriophages%20capable%20of%20controlling%20Pathogenic%20Vibrio%20bacteria%20in%20aquaculture.pdf; https://eprints.ums.edu.my/id/eprint/24583/

This study was conducted to analyze the complete genome sequence of two Vibriophages designated as VALLPKK3 and VPLPKKS. The Vibriophages VALLPKK3 and VPLPKKS have been shown to infect Vibrio alginolyticus and Vibrio parahaemolyticus, respectively. The analysis was acheived using various bioinformatics tools. The result revealed that the Vibriophage designated as VALLPKK3 was identified belonging to the T4-like Myoviridae. The VPLPKKS, however, was a novel Siphoviridae. The genome size of the VALLPKK3 was estimated at 248,088 bp with G+C content of 41.2%. It has about 390 open reading frames (orf) and the predicted coding sequences (eds) represented 92.4 % of the genome. Meanwhile, the genome size of the VALLPKKS was estimated to be at 56,637 bp with G+C content of 51.3%. The genome annotation revealed that the phage has about 80 open reading frames ( orf) and the predicted coding sequences (eds) shown to represent 90.7 % of its genome. All predicted coding sequences (eds) with known functions for both genomes were annotated as functional proteins for bacteriophage replication. The gene analysis revealed the absence of lysogeny related proteins in both phages. Although toxin search revealed the presence of a eds resembled to pilin gene in both genomes, it was difficult to verify at this stage that the phages harbored virulence factor because the gene could be used by the phages during host attachment and penetration. Interestingly, the genome of Vibriophage VPLPKKS is filled with new genes. It is predicted that Vibriophage VPLPKKS is likely to be a new member in the family of Siphoviridae. The findings of the present study could lead to a discovery of effective phages against fish bacterial pathogens via bacteriophage therapy in the future.