Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene
Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, m...
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my.ums.eprints.240912019-11-14T06:00:54Z https://eprints.ums.edu.my/id/eprint/24091/ Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene Nazia Abdul Kadar Sitti Raehanah Muhamad Shaleh Emma Suali QK Botany SH Aquaculture. Fisheries. Angling Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp. (99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species levels. 2018 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/24091/1/Molecular%20and%20Phylogenetic%20Identification%20of%20Marine%20Microalgae%20Inferred%20by%2018S%20rDNA%20Gene.pdf Nazia Abdul Kadar and Sitti Raehanah Muhamad Shaleh and Emma Suali (2018) Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene. Malays. Appl. Biol., 47 (6). pp. 41-45. |
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QK Botany SH Aquaculture. Fisheries. Angling Nazia Abdul Kadar Sitti Raehanah Muhamad Shaleh Emma Suali Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene |
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Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp. (99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species levels. |
format |
Article |
author |
Nazia Abdul Kadar Sitti Raehanah Muhamad Shaleh Emma Suali |
author_facet |
Nazia Abdul Kadar Sitti Raehanah Muhamad Shaleh Emma Suali |
author_sort |
Nazia Abdul Kadar |
title |
Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene |
title_short |
Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene |
title_full |
Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene |
title_fullStr |
Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene |
title_full_unstemmed |
Molecular and Phylogenetic Identification of Marine Microalgae Inferred by 18S rDNA Gene |
title_sort |
molecular and phylogenetic identification of marine microalgae inferred by 18s rdna gene |
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2018 |
url |
https://eprints.ums.edu.my/id/eprint/24091/1/Molecular%20and%20Phylogenetic%20Identification%20of%20Marine%20Microalgae%20Inferred%20by%2018S%20rDNA%20Gene.pdf https://eprints.ums.edu.my/id/eprint/24091/ |
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1760230195886817280 |
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13.188404 |