Isolation and characrerization of lead Pharmceutical metabolites of soft Coral (Order Alcyonacea)

• Main Author: Charles S. Vairappan
• Format: Research Report
• Language: English
• Published: Universiti Malaysia Sabah 2012
• Subjects: SH Aquaculture. Fisheries. Angling
• Online Access: https://eprints.ums.edu.my/id/eprint/23167/1/Isolation%20and%20characrerization%20of%20lead%20Pharmceutical%20metabolites%20of%20soft%20Coral.pdf; https://eprints.ums.edu.my/id/eprint/23167/

Soft corals of the class Octocorallia (order Alcyonacea) are well known as prolific organisms and they are popular among researchers as one of the important natural resources as many valuable metabolites were isolated from this marine organism. This study is about isolation and characterization of lead pharmace.utical metabolites of four main genera of soft corals; Sarcophyton, Lobophytum, Nephthea and Sinularia. The samples were collected from the three main coastal waters of Sabah; 1) Tunku Abdul Rahman Marine Park, 2) Tun Mustapha Marine Park, and 3) Tun Sakaran Marine Park. All the collected samples were extracted and profiled using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Fractions with interesting profile were further isolated and purified to get pure compounds. As a result, a total of 37 compounds of four chemical skeletons; cembranes, lobanes, sterols and germacrane were isolated. Isolated compounds were then subjected into bioassays including anti-microbial, cytotoxicity assay and photodynamic therapy assay. Most of cembranes and lobanes are active against E. coli, Salmonella typhii, S. enteritidis, S. thphymunium, Staphalococcus aereus and Vibrio cholera, at a concentration of 30 ~g. Cembranes also showed cytotoxicity against P-388 murine leaukemia cells and showed interesting activity against HL60 promyelocytic leukemia cells (from ATCC) in the photodynamic therapy assay.