Extraction of protein from microalgae using low pulsed electric field

Extraction of specific biological components from microalgae is often prevented by theintrinsic rigidity of the cell wall. Therefore, cell disruption is required to permit access to theinternal components of the cells. The purpose of this study is to extract protein from microalgae using low Pulsed...

Full description

Saved in:
Bibliographic Details
Main Authors: Salamah Samsu, Fouziah Md Yassin, Jedol Dayou
Format: Article
Language:English
Published: 2018
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/21991/1/Extraction%20of%20Protein%20from%20Microalgae%20Using%20Low%20Pulsed%20Electric%20Field.pdf
https://eprints.ums.edu.my/id/eprint/21991/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.21991
record_format eprints
spelling my.ums.eprints.219912019-05-08T06:29:14Z https://eprints.ums.edu.my/id/eprint/21991/ Extraction of protein from microalgae using low pulsed electric field Salamah Samsu Fouziah Md Yassin Jedol Dayou S Agriculture (General) Extraction of specific biological components from microalgae is often prevented by theintrinsic rigidity of the cell wall. Therefore, cell disruption is required to permit access to theinternal components of the cells. The purpose of this study is to extract protein from microalgae using low Pulsed Electric Field (PEF). Chlorella vulgaris, Scenedesmus sp. and Chlorococcum sp. were treated with PEF (80 V/cm) in silver/silver and stainless-steel/stainless-steel parallel electrodes treatment chambers for 10 minutes. Treated samples went through solid/liquid separation by centrifugation and the supernatants were further analyzed by Fourier Transforminfrared (FTIR) spectroscopy. Results showed that overheating occurred at the negativeterminal of the electrode causing damages to cells during treatment. Low pulse duty cycle was able to reduce the overheating effect during PEF treatment. From FTIR spectra, cellulose and protein were detected in the supernatant, indicating that the cells were successfully lysed and some protein managed to diffuse out of the cells during the treatment. 2018 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/21991/1/Extraction%20of%20Protein%20from%20Microalgae%20Using%20Low%20Pulsed%20Electric%20Field.pdf Salamah Samsu and Fouziah Md Yassin and Jedol Dayou (2018) Extraction of protein from microalgae using low pulsed electric field. Akademi Sains Malaysia (ASM) Journal, 11 (2). pp. 106-111. ISSN 1823−6782
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Salamah Samsu
Fouziah Md Yassin
Jedol Dayou
Extraction of protein from microalgae using low pulsed electric field
description Extraction of specific biological components from microalgae is often prevented by theintrinsic rigidity of the cell wall. Therefore, cell disruption is required to permit access to theinternal components of the cells. The purpose of this study is to extract protein from microalgae using low Pulsed Electric Field (PEF). Chlorella vulgaris, Scenedesmus sp. and Chlorococcum sp. were treated with PEF (80 V/cm) in silver/silver and stainless-steel/stainless-steel parallel electrodes treatment chambers for 10 minutes. Treated samples went through solid/liquid separation by centrifugation and the supernatants were further analyzed by Fourier Transforminfrared (FTIR) spectroscopy. Results showed that overheating occurred at the negativeterminal of the electrode causing damages to cells during treatment. Low pulse duty cycle was able to reduce the overheating effect during PEF treatment. From FTIR spectra, cellulose and protein were detected in the supernatant, indicating that the cells were successfully lysed and some protein managed to diffuse out of the cells during the treatment.
format Article
author Salamah Samsu
Fouziah Md Yassin
Jedol Dayou
author_facet Salamah Samsu
Fouziah Md Yassin
Jedol Dayou
author_sort Salamah Samsu
title Extraction of protein from microalgae using low pulsed electric field
title_short Extraction of protein from microalgae using low pulsed electric field
title_full Extraction of protein from microalgae using low pulsed electric field
title_fullStr Extraction of protein from microalgae using low pulsed electric field
title_full_unstemmed Extraction of protein from microalgae using low pulsed electric field
title_sort extraction of protein from microalgae using low pulsed electric field
publishDate 2018
url https://eprints.ums.edu.my/id/eprint/21991/1/Extraction%20of%20Protein%20from%20Microalgae%20Using%20Low%20Pulsed%20Electric%20Field.pdf
https://eprints.ums.edu.my/id/eprint/21991/
_version_ 1760229912488181760
score 13.209306