β-Mannanase production by aspergillus flavus in solid-state fermentation of palm kernel cake
β-Mannanase production in batch solid-state fermentation (SSF) of palm kernel cake (PKC) was evaluated with flasks and a laterally aerated moving bed (LAMB) bioreactor using Aspergillus flavus UMS01. Optimum condition for flask SSF was 110 % moisture content, initial pH 6, 30 °C and particle size 85...
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| Main Authors: | , , , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
Springer, Boston, MA
2013
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/20364/1/%CE%B2.pdf https://eprints.ums.edu.my/id/eprint/20364/ https://doi.org/10.1007/978-1-4614-6208-8_48 |
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| Summary: | β-Mannanase production in batch solid-state fermentation (SSF) of palm kernel cake (PKC) was evaluated with flasks and a laterally aerated moving bed (LAMB) bioreactor using Aspergillus flavus UMS01. Optimum condition for flask SSF was 110 % moisture content, initial pH 6, 30 °C and particle size 855 μm, yielding 383 U g−1 dry PKC after 120 h. Under the same condition and particle size <5 mm, SSF in LAMB produced 276 U g−1 dry PKC at an optimal gas flow of 4.4 m s−1 in just 96 h. Optimal β-mannanase production was consistent with highest fungal growth and mannose production; to support increasing fungal growth, secretion of β-mannanase increased to degrade mannans in PKC, producing mannose for microbial consumption. A. flavus UMS01 showed promising attributes as a β-mannanase producer via SSF of PKC in flasks and LAMB bioreactor. |
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