Growth Characteristics and Production of Physalins from Physalis minima Hairy Roots in Shake Flasks

• Main Authors: Jualang Azlan Gansau, Marziah Mahmood
• Format: Article
• Language: English
• Published: Elsevier B.V. 2013
• Subjects: QK Botany
• Online Access: https://eprints.ums.edu.my/id/eprint/20297/1/Growth%20Characteristics%20and%20Production%20of%20Physalins%20from%20Physalis.pdf; https://eprints.ums.edu.my/id/eprint/20297/; http://kasetsartjournal.ku.ac.th/kuj_files/2013/A1311141130011562.pdf

This report described the growth characteristics and production of physalins from Physalis minima hairy roots in shake fl ask culture. The presence of lateral branches in the inoculum had a negligible effect on the fi nal root biomass dry weight (DW), specifi c growth rate (μ), doubling time (td) and production of physalins. However, excising the primary and lateral root tips reduced μ and the total root length but not the fi nal biomass. Mature root tissues were observed to accumulate more physalin B and F (1.55 and 3.74 mg.g-1 DW, respectively) compared to the root tips (0.65 and 1.47 mg.g-1 DW, respectively). Increasing the number of root tips from 2 to 12 and the medium volume signifi cantly reduced μ and extended td. Decreasing the medium volume with a small number of inocula reduced td, improved the biomass and production of physalins and μ. Using a 100 mL fl ask, four root tips cultured in 25 mL medium provided the optimum conditions for biomass (0.24 g DW) and production of physalins (1.68–3.5 mg.g-1 DW).