Evaluation of acetylcholinesterase source from fish, Tor tambroides for detection of carbamate

• Main Authors: Siti Aqlima Ahmad, Mohd Khalizan Sabullah, Nor Aripin Shamaan, Mohd Yunus Abd. Shukor, Hussain Jirangon, Ariff Khalid, Mohd Arif Syed
• Format: Article
• Language: English
• Published: Triveni Enterprises 2016
• Subjects: QH301-705.5 Biology (General)
• Online Access: https://eprints.ums.edu.my/id/eprint/19626/1/Evaluation%20of%20acetylcholinesterase%20source%20from%20fish.pdf; https://eprints.ums.edu.my/id/eprint/19626/; http://www.jeb.co.in/journal_issues/201607_jul16/paper_01.pdf

Acetylcholinesterase (AChE) from the brain tissue of local freshwater fish, was isolated through affinity purification. Acetylthiocholine iodide (ATCi) was preferable synthetic substrate to purified AChE with highest maximal velocity (V ) and lowest biomolecular constant (K ) at 113.60 Umg and 0.068...