Screening of preliminary biological activities and In-Vitro cultures pereskia bleo and rennellia elliptica

• Main Author: Aina Syafiqah Abdullah
• Format: Thesis
• Language: English
• Published: 2010
• Subjects: QD Chemistry
• Online Access: https://eprints.ums.edu.my/id/eprint/19338/1/Screening%20of%20preliminary%20biological%20activities%20.pdf; https://eprints.ums.edu.my/id/eprint/19338/

Pereskia bIeo has been used by the local community of Malaysia to treat diabetes, hypertension, anti-tumor, anti-rheumatic, anti-ulcer and anti-inflammatory, while Rennellia elliptica were used to enhance inner strength, post natal treatment and to relief joints pain. 5aeening of phytod1emicals, antibacterial, antifungal and Inhibitor for protein phosphatase type 1, MAP kinase and MAP kinase phosphatase were performed to evaluate the medicinal properties of these plants. Bioassay through agar diffusion method shows that moderately polar extracts (MPE) for both plants were biologically active. The MPE of old leaves of P. bIeo shows the best Inhibitory activities against s. typhi (23.00±0.50 mm), protein phosphatase type 1 (14.17±1.26 mm), MAPK kinase (33.33±1.44 mm), and MAPK kinase phosphatase (10.50±0.50 mm). The MPE of the leaves of R. elliptica shows the largest inhibition on P. vulgaris (17.33±0.76 mm), while the MPE of the stems shows the best inhibitory activities against MAPK kinase (16.50%1.32 mm), and MAPK kinase phosphatase (9.33%0.58 mm). In vitro seeds germination of P. bleo was 100% germination using Murashige and Skoog medium (MS), Bs medium and Vacin and Went medium. Callus induction from the seed of P. bleo using MS medium supplemented with 2.5 mg/L 2,+0 and 1.5 mg/L kinetin give the highest callus fresh weight (2.9833±0.1302 g). The root explants of R. elliptica formed callus on MS medium supplemented with 0.4 mg/l to 1.0 mg/L 2,+0. Roots Induction from the root-derived callus was accomplished on MS medium supplemented with 2.0 mg/L lBA and 0.2 mg/LBAP.