Quantification of phytochemical contents and antimicrobial activity of Plectocomiopsis Geminiflora

This study was carried out at the Faculty of Sustainable Agriculture, Universiti Malaysia Sabah (UMS) Sandakan Campus from July 2015 to September 2015 to study the effect of different extraction solvents on the extraction yield, qualitative and quantitative phytochemical contents and antimicrobial...

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Bibliographic Details
Main Author: Aiqueenelyn Jites
Format: Academic Exercise
Language:English
Published: 2016
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/18895/1/Quantification%20of%20phytochemical%20contents%20and%20antimicrobial%20activity%20of%20Plectocomiopsis%20Geminiflora.pdf
https://eprints.ums.edu.my/id/eprint/18895/
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Summary:This study was carried out at the Faculty of Sustainable Agriculture, Universiti Malaysia Sabah (UMS) Sandakan Campus from July 2015 to September 2015 to study the effect of different extraction solvents on the extraction yield, qualitative and quantitative phytochemical contents and antimicrobial activity of Plectocomiopsis geminiflora. Aqueous ethanol used for extraction solvent showed the highest extraction yield as compared to aqueous methanol and distilled water. For the qualitative analysis, distilled water extract indicates more presence of phytochemicals as compare to aqueous ethanol and aqueous methanol. From this analysis, the result indicates the presence of saponins, glycosides, flavonoids, sterOid, phenols, alkaloids, tannins and terpenoids and the absence of quinones and anthocyanin. For the quantitative analysis, distilled water extract showed the highest total of phenoliC and alkaloid content while for flavonoid content, methanol extract showed highest amount. The antimicrobial activity which was focused only into the antibacterial activity has been done using disc diffusion technique. From this experiment, it revealed that only Bacillus cereus was susceptible to all three extraction solvents while others bacteria such as Eschoria coli, Salmonella thypii, and Staphylococcus aureus only susceptible to distilled water extract. This indicates the potential usefulness of this plant in the treatment of various pathogenic diseases in this locality.