Comparison on the quality of local village cockerel sperm using four different diluents at room temperature

• Main Author: Loo, Hua Bin
• Format: Academic Exercise
• Language: English
• Published: 2016
• Subjects: S Agriculture (General)
• Online Access: https://eprints.ums.edu.my/id/eprint/18816/1/Comparison%20on%20the%20quality%20of%20local%20village%20cockerel%20sperm%20using%20four%20different%20diluents%20at%20room%20temperature.pdf; https://eprints.ums.edu.my/id/eprint/18816/

A study was carried out at the Faculty of Sustainable Agriculture (FSA), Sandakan Campus, Universiti Malaysia Sabah (UMS) from August until November 2015. The purpose of this study was to evaluate the coconut water (CW) as natural diluents against chemical diluents for cockerel semen preservation at room temperature (25°C). Four out of 30 local village cockerels were selected for semen collection. Semen collection was repeated twice a week in pooled semen for three replicates of the fresh pooled semen samples with a minimum of 85% individual forward motility. Fresh semen quality was observed based on semen volume, colour and consistency, concentration, mass motility (MMOT) and individual forward motility. Mean volume of the pooled semen was 0.24±0.01 mL, mean colour and consistency of pooled semen was 4.67±0.58 and mean concentration of fresh pooled semen was 1.37±0.02x109 sperm/ mL. Moreover, mean MMOT and individual forward motility of fresh pooled semen were 5.67±0.58 and 90.0±0% respectively. Pooled semen samples with more than 85% of individual forward motility were diluted with four different semen diluents, which were Ringer's solution, TRIS solution, old coconut water (CW) and young coconut water (CW). Pooled semen volume of 20 IJL was diluted with 680 IJL of the four different diluents in four 2 mL eppendoft tubes separately. This experiment was conducted in the laboratory over a 12 hour intervals after semen collection. Data were taken every hour from four eppendoft tubes for individual forward motility, percentage of live sperm, percentage of head, neck and tail abnormalities and total sperm abnormalities. Ringer's solution showed the best result in comparison to the other three semen cockerel diluents. It does not showed any significant different (p>0.05) but it showed the highest individual forward motility (46.67±25.17%) in comparison with old CW (6.67±5.77%) at the 12th hour of observation. Ringer's solution (16.67±1.53%) showed there were significant differences (p<O.Ol) for live sperm percentage in comparison with old CW (9.33±2.08%) at the same hour of observation. Both of the solutions showed an unstable trend of sperm abnormalities percentage over a 12 hour of intervals. However, TRIS solution and young CW showed a poorer effect on individual forward motility and live sperm percentage where the sperm only survived up to 9th and 5th hour respectively. It was concluded that old CW was comparable to Ringer's solution and it can be an alternative source for cockerel semen diluents as natural diluents as it is cheaper and readily available all year round in Malaysia and other tropical country.