Type and amount of glucosinolates in the leaves of Moringa Oleifera

• Main Author: Monjia Belleza Cosmas Mojulat
• Format: Academic Exercise
• Language: English
• Published: 2014
• Subjects: QH Natural history; QH301-705.5 Biology (General)
• Online Access: https://eprints.ums.edu.my/id/eprint/17534/1/Type%20and%20amount%20of%20glucosinolates%20.pdf; https://eprints.ums.edu.my/id/eprint/17534/

"Glucosinolatesa nd their hydrolysis product isothiocyanatesa re actively researched due to their anti cancer properties as well as their high potential usage in the biomedical field. This study was done to detect and identify the presence of glucosinolates, particularly the glucomoringin (GMG), in the leaves of the locally grown Moringa oleifera by identifying its derived isothiocyanate, glucomoringin isothiocyanate (GMG-ITC). The hydrolysis was carried out in optimum condition resulting in the presence of isothiocyanate which was subsequently extracted using dichloromethane.C onsequently,t he identificationa nd analysiso f the isothiocyanate was conducted using Gas Chromatography-MassS pectrometry (GC-MS). Besides that, this study was also done to quantify the amount of glucosinolate identified in the leaves of M. oleifera. Quantification of the amount of glucosinolate present was done using the High Performance Liquid Chromatography (HPLC) method. The GCMS results showedt he occurrenceo f a minor isothiocyanate,b enzyl isothiocyanatein the sample extracts. This observation proves the presence of a minor glucosinolate, benzyl glucosinolate in the leaves sample of the locally grown M. oleifera. However, the GC-MS was unable to identify GMG-ITC due to its low volatility. On the other hand, result of HPLC proves the presence of minor glucosinolate, benzyl glucosinolate in the M. oleifera leaves sample with the amount of 0.3154 g. Even though future research is needed to confirm these findings, this study helps provide a preliminary database on the glucosinolates present in the locally grown M. oleifera which can contribute to the biomedical field as well as the local production of pharmaceuticals products.