Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)

• Main Authors: Chew, Few Ne, Tan, Siang Wen, Boo, Chern Huey, Tey, Ti Beng
• Format: Article
• Published: Taylor & Francis Group 2012
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/9310/; http://dx.doi.org/10.1080/10826068.2012.660903

An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial d...