Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach

Volatile components have been identified in hundreds of orchids including species and hybrids, but the biochemical and molecular biological knowledge on the biosynthesis of scent compounds in orchids is still limited. To date, a strategy combining chemical analysis, genomics and bioinformatics is ad...

Full description

Saved in:
Bibliographic Details
Main Authors: Barudin, Mohd Aiman, Mohd Hairul, Ab. Rahim, Tan, Suat Hian
Format: Article
Language:English
Published: International Journal of Interdisciplinary and Multidisciplinary Studies 2015
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/8797/1/Identification%20of%20Fragrance-Related%20Transcripts%20from%20Selected%20Orchids%20Using%20cDNA%20Representational%20Difference%20Analysis%20%28cDNA-RDA%29%20Approach.pdf
http://umpir.ump.edu.my/id/eprint/8797/
http://www.ijims.com/uploads/0ca08b89804dc723a444hairul25.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ump.umpir.8797
record_format eprints
spelling my.ump.umpir.87972018-06-29T01:58:04Z http://umpir.ump.edu.my/id/eprint/8797/ Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian Q Science (General) Volatile components have been identified in hundreds of orchids including species and hybrids, but the biochemical and molecular biological knowledge on the biosynthesis of scent compounds in orchids is still limited. To date, a strategy combining chemical analysis, genomics and bioinformatics is adopted to unravel the scent biosynthetic pathways that are involved in floral scent biosynthesis in orchids. In this study, cDNA-RDA was used to identify fragrance-related transcripts in three selected fragrant-orchids includingVanda Mimi Palmer, Vanda Small Boy Leong and Vandachostylis Sri-Siamin comparison to Vanda Tan Chay Yan, a nonfragrant orchid. This cDNA-RDA approach allows us toisolate cDNA fragments that are presentin selected fragrant orchids but absent in non-fragrant orchid. Double-stranded cDNA of each fragrant orchid was hybridized separately with double-stranded cDNA of the selected non-fragrant orchid and followed by amplification of subtracted fragments. Three rounds of cDNA-RDAwere carried out to enrich difference cDNA fragments.A number of 21 clones of difference products were sequenced and sequence analysis has shown the presence of three different putative fragrance-related transcripts including methionine synthase, S-adenosyl-Lmethionine synthase, and phenylalanine ammonia lyase. All of the identified transcripts might be involved in fragrance biosynthesis of those fragrant orchids International Journal of Interdisciplinary and Multidisciplinary Studies 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8797/1/Identification%20of%20Fragrance-Related%20Transcripts%20from%20Selected%20Orchids%20Using%20cDNA%20Representational%20Difference%20Analysis%20%28cDNA-RDA%29%20Approach.pdf Barudin, Mohd Aiman and Mohd Hairul, Ab. Rahim and Tan, Suat Hian (2015) Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach. International Journal of Interdisciplinary and Multidisciplinary Studies, 2 (4). pp. 198-207. ISSN 2348 – 0343 http://www.ijims.com/uploads/0ca08b89804dc723a444hairul25.pdf
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Barudin, Mohd Aiman
Mohd Hairul, Ab. Rahim
Tan, Suat Hian
Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
description Volatile components have been identified in hundreds of orchids including species and hybrids, but the biochemical and molecular biological knowledge on the biosynthesis of scent compounds in orchids is still limited. To date, a strategy combining chemical analysis, genomics and bioinformatics is adopted to unravel the scent biosynthetic pathways that are involved in floral scent biosynthesis in orchids. In this study, cDNA-RDA was used to identify fragrance-related transcripts in three selected fragrant-orchids includingVanda Mimi Palmer, Vanda Small Boy Leong and Vandachostylis Sri-Siamin comparison to Vanda Tan Chay Yan, a nonfragrant orchid. This cDNA-RDA approach allows us toisolate cDNA fragments that are presentin selected fragrant orchids but absent in non-fragrant orchid. Double-stranded cDNA of each fragrant orchid was hybridized separately with double-stranded cDNA of the selected non-fragrant orchid and followed by amplification of subtracted fragments. Three rounds of cDNA-RDAwere carried out to enrich difference cDNA fragments.A number of 21 clones of difference products were sequenced and sequence analysis has shown the presence of three different putative fragrance-related transcripts including methionine synthase, S-adenosyl-Lmethionine synthase, and phenylalanine ammonia lyase. All of the identified transcripts might be involved in fragrance biosynthesis of those fragrant orchids
format Article
author Barudin, Mohd Aiman
Mohd Hairul, Ab. Rahim
Tan, Suat Hian
author_facet Barudin, Mohd Aiman
Mohd Hairul, Ab. Rahim
Tan, Suat Hian
author_sort Barudin, Mohd Aiman
title Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_short Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_full Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_fullStr Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_full_unstemmed Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_sort identification of fragrance-related transcripts from selected orchids using cdna representational difference analysis (cdna-rda) approach
publisher International Journal of Interdisciplinary and Multidisciplinary Studies
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/8797/1/Identification%20of%20Fragrance-Related%20Transcripts%20from%20Selected%20Orchids%20Using%20cDNA%20Representational%20Difference%20Analysis%20%28cDNA-RDA%29%20Approach.pdf
http://umpir.ump.edu.my/id/eprint/8797/
http://www.ijims.com/uploads/0ca08b89804dc723a444hairul25.pdf
_version_ 1643665968754327552
score 13.18916