Feasibility study on locally isolated strain for butanol production by using oil palm trunk sap

• Main Author: Asmadiyana, Mahadi
• Format: Undergraduates Project Papers
• Language: English
• Published: 2013
• Subjects: TD Environmental technology. Sanitary engineering
• Online Access: http://umpir.ump.edu.my/id/eprint/7230/1/Feasibility_study_on_locally_isolated_strain_for_butanol_production.pdf; http://umpir.ump.edu.my/id/eprint/7230/

As the rising price of crude oil become unstoppable crisis and the shortage of resources start to fear people, the demanding of non-renewable resources gradually incline each days. The production of biobutanol which having numbers of advantages and have potential to replace the uses of petrochemical played a very important role. In biobutanol production, not only the biomass for the carbon sources in fermentation process but the microbes used also must be very precise. In order to improve the current production, the study on locally isolated strains to produce biobutanol by using local plant is proposed. The strain is isolated from soil sample taken at oil palm plantation before proceed with the fermentation process to produce biobutanol. The isolation process is done under strict anaerobic condition since the potential butanol producer strain generally is an anaerobic bacteria. For the fermentation process oil palm trunk sap is used as the carbon sources to produce the biobutanol at 37oC for 5 days. From this research, there are three isolated bacteria which are; isolate 1a, isolate 2b and isolate 3b, which had been identified, can produce biobutanol and other biosolvents. Among the three of the isolated bacteria, isolate 2b was identified to be the best isolated strain in production of butanol and other biosolvents with 5.41 g/L of butanol, 15.04 g/L of acetone and 5.90 g/L of ethanol.