Screening of xylanase producer from soil

• Main Author: Wan Raimie, Wan Ramli
• Format: Undergraduates Project Papers
• Language: English
• Published: 2008
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/669/1/WAN_RAIMIE_BINTI_WAN_RAMLI.pdf; http://umpir.ump.edu.my/id/eprint/669/

The purpose of this study is to screen and to characterize the bacterial producing xylanase. The scopes include screening of xylanase producer and characterization of microorganisms and analysis of enzyme. This research was about an alternative to produce xylanase instead of using agricultural and forestry wastes, paper industry wastes, and various fruit wastes. The screening procedure was started from collecting soil sample until growth of bacteria on selective agar plate to screen the best xylanase producer from sample. After that, xylanase producer was characterized based on morphology using four types of morphological test. Besides that, the enzyme needs to be analyzed in different pH and temperature in term of protein content and xylanase activity. The optimum xylanase activity obtained was 72.667 U/ml at pH 6 and 65˚C.