Application of Adsorption Chromatography in the Papain Processing
A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow v...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Global Science and Technology Forum
2014
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf http://umpir.ump.edu.my/id/eprint/6260/ https://www.globalsciencejournals.com/article/10.7603%2Fs40837-014-0002-8 |
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| Summary: | A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice. |
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