Application of calcined kaolin as support for the immobilization of lipase from candida rugosa

• Main Author: Shahrul Nazmi, Othman
• Format: Undergraduates Project Papers
• Language: English
• Published: 2008
• Subjects: QP Physiology
• Online Access: http://umpir.ump.edu.my/id/eprint/626/1/32.Application%20of%20calcined%20kaolin%20as%20support%20for%20the%20immobilization%20of%20lipase%20from%20candida%20rugosa.PDF; http://umpir.ump.edu.my/id/eprint/626/

Lipase from Candida rugosa was immobilized onto calcined-kaolin by physical adsorption method. Lipase offers wide ranges of applications; where as the ability of lipase to act in both organic and inorganic solvents making it more valuable in term of application. However the major concern still will be the cost of lipase, the availability of lipase and reusability of lipase. In order to curb the problems, immobilization of lipase is becoming normal practices. Kaolin is natural substance that obtained from clay. With abilities to act as good as ceramic material; (no toxicity, ability to withstand extreme heat and pressure) it is believed that kaolin has tremendous potential to be utilized as solid support material in both laboratory scale and industrial scale. The study was focus on the preparation of treated kaolin to utilize natural resources instead of inorganic substances. The objectives of this study are to study, amount of lipase attach to kaolin as support material in form of immobilized lipase, to record performance of immobilized lipase with the effect of temperature and effect of pH. The last objective is to compare enzyme activity between free lipase and immobilized lipase. It is determined that percentage of protein loading is 68% (relative amount of lipase attached to solid support material).