Batch extraction of 1,3,7-trymethylxanthine from KKM22 cocoa seeds

• Main Author: Norhamizah, Johari
• Format: Undergraduates Project Papers
• Language: English
• Published: 2012
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/4378/1/15.Batch%20extraction%20of%201%2C3%2C7-trymethylxanthine%20from%20KKM22%20cocoa%20seeds.pdf; http://umpir.ump.edu.my/id/eprint/4378/

1,3,7- trymethylxanthine (caffeine) is a naturally occurring substance found in cocoa seeds.The purpose of this research is to extract caffeine from Klon Koko Malaysia (KKM)22, to investigate the effect of particle size,solvent/feed ratio and extraction time,and also to determine the kinetic model for extraction of 1,3,7-trymethylxantine.The sample was prepared by grinding and sieving, followed by solid-liquid extraction using water by heat reflux extracting technique, liquid-liquid extraction with ethyl acetate,drying of caffeine by rotary evaporator,and finally analysis of the caffeine yield.The analysis of the caffeine yield was done using UV/Vis Spectrophotometric method.The caffeine yield was highest at sample particle size of 315 μm (0.22 % w/w caffeine or 2.1724 mg/g cocoa),solvent/feed ratio of 1:1 (0.22 % w/w caffeine or 2,2102 mg/g cocoa),and extraction time of 90 minutes (0.23 % w/w caffeine or 2.288 mg/g cocoa).The best conditions for the highest yield of caffeine from KKM22 were 315 μm of sample particle size, 1:1 of solvent/feed ratio, and 90 minutes of extraction time.