qPCR analysis of quorum sensing genes of pseudomonas aeruginosa: Primer design and qPCR efficiency

• Main Authors: Saiedatul Akmal, Mohamed Sedek, Mohd Azmir, Arifin, Mimi Sakinah, A. M.
• Format: Article
• Language: English
• Published: Penerbit Universiti Malaysia Pahang 2023
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/37835/1/qPCR%20Analysis%20of%20Quorum%20Sensing%20Genes%20of%20Pseudomonas%20aeruginosa.pdf; http://umpir.ump.edu.my/id/eprint/37835/; https://doi.org/10.15282/ijets.10.1.2023.1006; https://doi.org/10.15282/ijets.10.1.2023.1006

A well-designed and properly validated primer produces a specific and efficient qPCR assay. The primer’s melting temperature, G-C content, its size, as well as the amplicon’s size are the principal considerations while designing the primer for this study and it was in accordance with the MIQE guidel...