Evaluation of antioxidant, antibacterial and anticancer activities of ganoderma lucidum extracts

• Main Authors: N. I., Azahar, Swan, Sia Yiik, Nur Syahirah, Mohd Razi, Zhen, Wan Yan, Mohd Azmir, Arifin
• Format: Conference or Workshop Item
• Language: English
• Published: 2022
• Subjects: QD Chemistry; T Technology (General); TA Engineering (General). Civil engineering (General); TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/36880/1/Evaluation%20of%20antioxidant%2C%20antibacterial%20and%20anticancer%20activities%20of%20ganoderma%20lucidum%20extracts.pdf; http://umpir.ump.edu.my/id/eprint/36880/; https://ncon-pgr.ump.edu.my/index.php/en/?option=com_fileman&view=file&routed=1&name=E-BOOK%20NCON%202022%20.pdf&folder=E-BOOK%20NCON%202022&container=fileman-files

G. lucidum is an oriental fungus loaded with antioxidant, antimicrobial and anticancer properties. G. lucidum extracts obtained by using soxhlet and Ultrasonic-Assisted Extraction (UAE). The antioxidant activity determined by DPPH assay resulting in IC50 value of extract for soxhlet water 372.21μg/mL followed by soxhlet ethanol 431.00μg/mL, UAE ethanol 541μg/mL and UAE water 560.90μg/mL shown weak antioxidant properties. Well plate diffusion used for antimicrobial activity test against E. coli and S. aureus. The UAE water extract shown highest antibacterial activity against S. aureus (20-23) mm followed by soxhlet water extract (6-13) mm. The ethanol extract for both soxhlet and UAE are (5-13) mm and (4-14) mm respectively. G. lucidum extract exhibited zero inhibition zone against E. coli due to presence of barrier membrane. CCK-8 used to test anticancer activity against MCF-7 cells. The IC50 values of soxhlet ethanolic extract is 4.797 μg/mL followed by UAE ethanolic extract 5.291 μg/mL, soxhlet water extract 7.196 μg/mL and UAE water extracts 9.455 μg/mL. The lower IC50 value indicated that the extracts inhibited cell viability of MCF-7.