Antidiabetic Effect of Actinodaphne angustifolia and Profiling of Bioactive Metabolites using UPLC-QToF/ESI-MS Method

• Main Authors: F., Md. Shaekh, W. I, Wan Maznah, M. A., Mohd Fadhlizil Fasihi, A., Shihab Uddin, R., Kamrul, T., Saiful Nizam, H., AKM Moyeenul
• Format: Article
• Language: English
• Published: Faculty of Medicine, Universiti Kebangsaan Malaysia 2023
• Subjects: QD Chemistry; R Medicine (General)
• Online Access: http://umpir.ump.edu.my/id/eprint/36755/1/Antidiabetic%20Effect%20of%20Actinodaphne%20angustifolia.pdf; http://umpir.ump.edu.my/id/eprint/36755/; https://doi.org/10.17576/MH.2023.1801.12; https://doi.org/10.17576/MH.2023.1801.12

Diabetes mellitus adalah gangguan kronik yang menyebabkan paras glukosa darah meningkat akibat kekurangan insulin, sama ada sepenuhnya atau sebahagian. Kami menyiasat sifat antidiabetik Actinodaphne angustifolia dalam model tikus dan mengenal pasti fitokimia bioaktif dengan menggunakan kaedah UPLC-QTOF/ ESI-MS. Struktur pankreas tikus, profil lipid dan glukosa darah dinilai selepas intervensi selama satu minggu. Analisis UPLC-QTOF/ESI-MS telah dijalankan untuk mengenal pasti flavonoid dan terpenoid dalam ekstrak daun. Ekstrak Actinodaphne angustifolia dengan ketara meningkatkan lipoprotein berketumpatan tinggi (HDL) (p<0.05) sambil mengurangkan jumlah kolesterol (TC), lipoprotein berketumpatan rendah (LDL) dan glukosa darah. Tambahan pula, struktur seni tisu pulau pankreas juga pulih dengan baik berbanding kumpulan kawalan. Sebanyak 45 flavonoid dan 109 sebatian terpenoid telah dikenal pasti menggunakan analisis berasaskan UPLC-QTOF/ESI-MS dan kajian tambahan perlu dijalankan untuk mengenal pasti agen antidiabetik yang berpotensi. Diabetes mellitus is a chronic disorder that causes elevated blood glucose levels due to a lack of insulin, either completely or partially. We investigated the antidiabetic property of Actinodaphne angustifolia in a rat model and identified the bioactive phytochemicals by using the UPLC-QTOF/ESI-MS method. The rats’ pancreatic structures, lipid profile and blood glucose were assessed after a one-week intervention. UPLC-QTOF/ESI-MS analysis was conducted to identify flavonoids and terpenoids in the leaf extract. Actinodaphne angustifolia extract markedly increased the high-density lipoprotein (HDL) (p<0.05) while reducing total cholesterol (TC), low-density lipoprotein (LDL) and blood glucose. Furthermore, the tissue architecture of pancreatic islets was also well recovered as compared to the control group. A total of 45 flavonoids and 109 terpenoid compounds were identified using UPLC-QTOF/ESI-MS-based analysis and additional studies should be undertaken to identify the potential antidiabetic agents.