Effects of variables on bipolymer production in 20l batch stirred tank fermentations

This research is conducted to study the effects of variables on polyhydroxybutyrate(PHB) production in 20L batch stirred tank fermentations by using Cupriavidus necator. It consist of three major phases starting with scale up process, followed by fermentation process and mathematical analysis. The f...

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Main Author: Mohd Firdaus, Hashim
Format: Undergraduates Project Papers
Language:English
Published: 2010
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Online Access:http://umpir.ump.edu.my/id/eprint/3387/1/CD5886_Firdaus_Hashim.pdf
http://umpir.ump.edu.my/id/eprint/3387/
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spelling my.ump.umpir.33872021-07-05T04:42:54Z http://umpir.ump.edu.my/id/eprint/3387/ Effects of variables on bipolymer production in 20l batch stirred tank fermentations Mohd Firdaus, Hashim TP Chemical technology This research is conducted to study the effects of variables on polyhydroxybutyrate(PHB) production in 20L batch stirred tank fermentations by using Cupriavidus necator. It consist of three major phases starting with scale up process, followed by fermentation process and mathematical analysis. The fermentation process is scaled up from the optimum condition in 500mL shake flask to 20L stirred tank bioreactor by approximating the set point values of air flowrate and agitation speed which gives the same dissolve oxygen tension (DOT) curve versus time. The closest DOT curve pattern to that of the optimum condition in 500mL shake flask is achieved by the combination 230 RPM agitation speed and 10L/min compressed air flowrate. This experiment is followed with fermentatin process involving the manipulation of the parameters agitation speed, temperature and initial glucose concentration. The 23 factorial experiment method is used to design the experiment around the set points of agitation speed and air flowrate which gave the optimum DOT curve. Among the experimental points, it is found that the highest yield of PHB is produced in condition of 210RPM agitation speed, 32! temperature and 23g/L initial glucose concentration. This experiment was followed by mathematical analysis using Yates’ Method to study the main effect and interactive effect of changing the level of experimental variables. The calculation proved that temperature has the biggest main effect on PHB production while the biggest interactive effect is from the combination of agitation speed and initial glucose concentration. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3387/1/CD5886_Firdaus_Hashim.pdf Mohd Firdaus, Hashim (2010) Effects of variables on bipolymer production in 20l batch stirred tank fermentations. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang .
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd Firdaus, Hashim
Effects of variables on bipolymer production in 20l batch stirred tank fermentations
description This research is conducted to study the effects of variables on polyhydroxybutyrate(PHB) production in 20L batch stirred tank fermentations by using Cupriavidus necator. It consist of three major phases starting with scale up process, followed by fermentation process and mathematical analysis. The fermentation process is scaled up from the optimum condition in 500mL shake flask to 20L stirred tank bioreactor by approximating the set point values of air flowrate and agitation speed which gives the same dissolve oxygen tension (DOT) curve versus time. The closest DOT curve pattern to that of the optimum condition in 500mL shake flask is achieved by the combination 230 RPM agitation speed and 10L/min compressed air flowrate. This experiment is followed with fermentatin process involving the manipulation of the parameters agitation speed, temperature and initial glucose concentration. The 23 factorial experiment method is used to design the experiment around the set points of agitation speed and air flowrate which gave the optimum DOT curve. Among the experimental points, it is found that the highest yield of PHB is produced in condition of 210RPM agitation speed, 32! temperature and 23g/L initial glucose concentration. This experiment was followed by mathematical analysis using Yates’ Method to study the main effect and interactive effect of changing the level of experimental variables. The calculation proved that temperature has the biggest main effect on PHB production while the biggest interactive effect is from the combination of agitation speed and initial glucose concentration.
format Undergraduates Project Papers
author Mohd Firdaus, Hashim
author_facet Mohd Firdaus, Hashim
author_sort Mohd Firdaus, Hashim
title Effects of variables on bipolymer production in 20l batch stirred tank fermentations
title_short Effects of variables on bipolymer production in 20l batch stirred tank fermentations
title_full Effects of variables on bipolymer production in 20l batch stirred tank fermentations
title_fullStr Effects of variables on bipolymer production in 20l batch stirred tank fermentations
title_full_unstemmed Effects of variables on bipolymer production in 20l batch stirred tank fermentations
title_sort effects of variables on bipolymer production in 20l batch stirred tank fermentations
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3387/1/CD5886_Firdaus_Hashim.pdf
http://umpir.ump.edu.my/id/eprint/3387/
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score 13.209306