D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation

• Main Authors: Nurul Atikah, Mohd Rodzri, Salwani, Md Zain, Rafidah, Mohamed Ali Hanapiah, Rozaimi, Abu Samah, R. M., Illias
• Format: Conference or Workshop Item
• Language: English
• Published: IOP Publishing 2020
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/29359/1/26.%20D-Xylonic%20acid%20from%20recombinant%20e.%20coli%20BL21%20%28DE3%29.pdf; http://umpir.ump.edu.my/id/eprint/29359/

Production ᴅ-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and production of ᴅ-xylonic acid in both shake flask and bioreactor was investigated. Higher specific growth rate of recombinant E. coli BL21 (DE3) and concentration of ᴅ-xylonic acid however, was obtained by fermentation in shake flask which is 0.273 h-1 and 9.06 gL-1 ᴅ-xylonic acid respectively. The result suggests several mistakes done during fermentation in bioreactor that causes low concentration of ᴅ-xylonic acid in bioreactor fermentation. Therefore, this calls up for improvement and better scale-up strategies in future work.