In vitro evaluation of Cuscuta reflexa Roxb. forthrombolytic, antioxidant, membrane stabilizingand antimicrobial activities

• Main Authors: Azad, A. K., Laboni, Farhina Rahman, Rashid, Harun, Ferdous, Sahena, Rashid, Shah Samiur, Nurkhalida, Kamal, Labu, Zubair Khalid, Islam, M. S., Sarker, Zaidul Islam
• Format: Article
• Language: English
• Published: Taylor & Francis 2018
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/24086/1/In%20vitro%20evaluation%20of%20Cuscuta%20reflexa%20Roxb1.pdf; http://umpir.ump.edu.my/id/eprint/24086/; https://doi.org/10.1080/14786419.2018.1538216; https://doi.org/10.1080/14786419.2018.1538216

The key purpose of this experiment was to evaluate the thrombolytic, antioxidant, membrane stabilizing and antimicrobial potentials of crude ethanol extracts (CEE) of whole plant, organic and aqueous soluble fractions (OF & AQSF). CEE showed the highest (44.63%) clot lysis activity compared to streptokinase (64.35%). In DPPH study, petroleum ether soluble fraction (PSF) has exhibited IC50 of 18.83 μg/mL while the standard ascorbic acid was 2.48 µg/mL. AQSF profoundly inhibited the lysis of erythrocytes (66.20%) which was insignificantly different (p > 0.05) to acetylsalicylic acid (71.98%), the reference. However, AQSF showed a significantly stronger level of protection against heat-induced hemolysis (64.80%) as compared with the acetylsalicylic acid (78.90%). CEE, OF and AQSF have displayed reasonable growth of inhibition of tested bacteria compared to negative control and standard drug (77.50 mg of GAE/g).