Optimization of vitexin and isovitexin compounds extracted from dried Mas Cotek leaves using one-factor-at-a-time (OFAT) approach in aqueous extraction

• Main Authors: Nasma, A., Aisyah, Naila, Azilah, Ajit, Ahmad Ziad, Sulaiman
• Format: Article
• Language: English
• Published: Penerbit Universiti Putra Malaysia 2018
• Subjects: Q Science (General)
• Online Access: http://umpir.ump.edu.my/id/eprint/23686/1/Aimi%20paper.pdf; http://umpir.ump.edu.my/id/eprint/23686/; http://www.ifrj.upm.edu.my/25%20(06)%202018/(43).pdf

Mas Cotek or Ficus deltoidea is regarded as one of the most precious herbal plants in Malaysia due to its content of beneficial active compounds and appreciation as a traditional remedy. This research investigated performance of aqueous or water extraction of dried Mas Cotek leaves for vitexin and isovitexin compounds using three regiments of temperatures (50, 70 and 100°C) in sample-to-water ratios of 1:10, 1:20 and 1:30g/mL during 8 hours of extraction. The optimum yield recorded for vitexin compound was 0.463 ± 0.045 (%w/w) from; 1:30 g/mL sampleto-water ratio, 50°C temperature at 4th hour of extraction. While for isovitexin compound, the optimum value recorded was 0.136 ± 0.015 (%w/w) from; 1:20 (g/mL) sample-to-water ratio, 50°C temperature at 5th hour of extraction. The morphological characterization of the extracted dried leaves particles performed using FE-SEM showed an intact and less damaged surface of extracted sample after 8 hours of extraction. The experimental values under best conditions were consistent with the predicted values, which suggested that aqueous extraction of Mas Cotek leaves is a good platform to serve as baseline study for more advanced extraction techniques.