Automatic Gram Staining for Sputum Slide

The Gram stain is the most important and universally used staining technique in the bacteriology laboratory. Gram staining method is used to do staining of the clinical material or the bacteria from colonies on laboratory media and provide a direct visualization of the morphology of the organisms ba...

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Main Authors: K. P., Ping, Rosyati, Hamid, Faradila, Naim, Nurul Wahidah, Arshad, Zeehaida , Mohamed
Format: Conference or Workshop Item
Language:English
English
Published: 2017
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/19455/1/Automatic%20Gram%20Staining%20for%20Sputum%20Slide.pdf
http://umpir.ump.edu.my/id/eprint/19455/2/Automatic%20Gram%20Staining%20for%20Sputum%20Slide%201.pdf
http://umpir.ump.edu.my/id/eprint/19455/
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spelling my.ump.umpir.194552018-02-08T07:06:20Z http://umpir.ump.edu.my/id/eprint/19455/ Automatic Gram Staining for Sputum Slide K. P., Ping Rosyati, Hamid Faradila, Naim Nurul Wahidah, Arshad Zeehaida , Mohamed TK Electrical engineering. Electronics Nuclear engineering The Gram stain is the most important and universally used staining technique in the bacteriology laboratory. Gram staining method is used to do staining of the clinical material or the bacteria from colonies on laboratory media and provide a direct visualization of the morphology of the organisms based on their reactions to the chemical present in stains. A sputum sample slide need to be staining before the quality of the sputum sample is determined. However, due to human inconsistency, some of the slides are heavily stain with dark color whereas some of it is lightly stained. This inconsistency would create a difficulty for automated sputum quality system using image processing. Therefore, an automated gram staining for sputum slide is needed in order to standardize the slide staining. The automated Gram-staining will undergo staining, washing, and drying process. Each process periods are controlled by a timer built in the microcontroller. The analysis is done on the accuracy of the position of the slide stain, the consistency of the amounts of staining solution drop on the slide and the time efficiency of this automated system is compared to manual operation. 2017 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19455/1/Automatic%20Gram%20Staining%20for%20Sputum%20Slide.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/19455/2/Automatic%20Gram%20Staining%20for%20Sputum%20Slide%201.pdf K. P., Ping and Rosyati, Hamid and Faradila, Naim and Nurul Wahidah, Arshad and Zeehaida , Mohamed (2017) Automatic Gram Staining for Sputum Slide. In: 4th International Conference On Electrical, Control And Computer Engineering (INECCE2017), 16-17 October 2017 , Hotel Adya, Langkawi. pp. 1-6.. (Unpublished)
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic TK Electrical engineering. Electronics Nuclear engineering
spellingShingle TK Electrical engineering. Electronics Nuclear engineering
K. P., Ping
Rosyati, Hamid
Faradila, Naim
Nurul Wahidah, Arshad
Zeehaida , Mohamed
Automatic Gram Staining for Sputum Slide
description The Gram stain is the most important and universally used staining technique in the bacteriology laboratory. Gram staining method is used to do staining of the clinical material or the bacteria from colonies on laboratory media and provide a direct visualization of the morphology of the organisms based on their reactions to the chemical present in stains. A sputum sample slide need to be staining before the quality of the sputum sample is determined. However, due to human inconsistency, some of the slides are heavily stain with dark color whereas some of it is lightly stained. This inconsistency would create a difficulty for automated sputum quality system using image processing. Therefore, an automated gram staining for sputum slide is needed in order to standardize the slide staining. The automated Gram-staining will undergo staining, washing, and drying process. Each process periods are controlled by a timer built in the microcontroller. The analysis is done on the accuracy of the position of the slide stain, the consistency of the amounts of staining solution drop on the slide and the time efficiency of this automated system is compared to manual operation.
format Conference or Workshop Item
author K. P., Ping
Rosyati, Hamid
Faradila, Naim
Nurul Wahidah, Arshad
Zeehaida , Mohamed
author_facet K. P., Ping
Rosyati, Hamid
Faradila, Naim
Nurul Wahidah, Arshad
Zeehaida , Mohamed
author_sort K. P., Ping
title Automatic Gram Staining for Sputum Slide
title_short Automatic Gram Staining for Sputum Slide
title_full Automatic Gram Staining for Sputum Slide
title_fullStr Automatic Gram Staining for Sputum Slide
title_full_unstemmed Automatic Gram Staining for Sputum Slide
title_sort automatic gram staining for sputum slide
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/19455/1/Automatic%20Gram%20Staining%20for%20Sputum%20Slide.pdf
http://umpir.ump.edu.my/id/eprint/19455/2/Automatic%20Gram%20Staining%20for%20Sputum%20Slide%201.pdf
http://umpir.ump.edu.my/id/eprint/19455/
_version_ 1643668656514662400
score 13.222552