Synthetic Curcumin Derivative DK1 Possessed G2/M Arrest and Induced Apoptosis Through Accumulation of Intracellular ROS in MCF-7 Breast Cancer Cells

• Main Authors: Norlaily, Mohd Ali, Swee, Keong Yeap, Nadiah, Abu, Kian, Lam Lim, Huynh, Ky, Ahmad Zaim, Mat Pauzi, Wan, Yong Ho, Sheau, Wei Tan, Han, Kiat Alan-Ong, Seema, Zareen, Noorjahan Banu, Alitheen, Akhtar, Muhammad Nadeem
• Format: Article
• Language: English
• Published: BioMed Central 2017
• Subjects: Q Science (General)
• Online Access: http://umpir.ump.edu.my/id/eprint/17120/1/fist-2017-nadeem-Synthetic%20curcumin%20derivative%20DK1.pdf; http://umpir.ump.edu.my/id/eprint/17120/; https://doi.org/10.1186/s12935-017-0400-3

Curcumin is a lead compound of the rhizomes of Curcuma longa and possess a broad range of pharmacological activities. Chemically, curcumin is 1,3-dicarbonyl class of compound, which exhibits keto-enol tautomerism. Despite of its strong biological properties, curcumin has yet been recommended as a therapeutic agent because of its poor bioavailability. A curcumin derivative (Z)-3-hydroxy-1-(2-hydroxyphenyl)-3-phenylprop-2-en-1-one (DK1) was synthesized and its cytotoxicity was tested on breast cancer cell MCF-7 and normal cell MCF-10A using MTT assay. Meanwhile, cell cycle regulation and apoptosis on MCF-7 cell were evaluated using flow cytometry. Regulation of cell cycle and apoptosis related genes expression was investigated by quantitative real time polymerase chain reaction (qRT-PCR), western blot and caspases activity analyses. Activation of oxidative stress on MCF-7 were evaluated by measuring ROS and GSH levels.