In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves

• Main Authors: Vijayalakshmi, A., Jayakumari, S., Masilamani, K.
• Format: Article
• Language: English; English
• Published: Penerbit Universiti Malaysia Pahang 2016
• Subjects: T Technology (General)
• Online Access: http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf; http://umpir.ump.edu.my/id/eprint/13806/7/ftech-2016-masilamani-Invitro%20antioxidant%20potential1.pdf; http://umpir.ump.edu.my/id/eprint/13806/; http://ijets.ump.edu.my/images/archive/Vol5/IJETS3.pdf

The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18 µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively. Index terms - Cassia tora, flavonoids, HPTLC, antioxidant