Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures

• Main Authors: Tan, Suat Hian, Maziah, Mahmood, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: eSAT Publishing House Pvt. Ltd. 2013
• Subjects: SB Plant culture
• Online Access: http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf; http://umpir.ump.edu.my/id/eprint/12672/; http://esatjournals.net/ijret/2013v02/i10/IJRET20130210037.pdf

The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e.250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16hphotoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size.