Comparison of Different Cell Disruption Methods And Cell Extractant Buffers For Recombinant Bromelain Expressed in E.COLI BL21-A1

• Main Authors: Zatul Iffah, Mohd Arshad, Azura, Amid, Muhd. Ezza Faiez, Othman
• Format: Article
• Language: English; English
• Published: Penerbit Universiti Teknologi Malaysia 2015
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/11859/1/Comparison%20Of%20Different%20Cell%20Disruption%20Methods%20And%20Cell%20Extractant%20Buffers%20For%20Recombinant%20Bromelain%20Expressed%20in%20E.COLI%20BL21-A1.pdf; http://umpir.ump.edu.my/id/eprint/11859/7/Comparison%20of%20Different%20Cell%20Disruption%20Methods%20and%20Cell%20Extractant%20Buffers%20for%20Recombinant%20Bromelain%20Expressed%20in%20E-coli%20BL21-A1.pdf; http://umpir.ump.edu.my/id/eprint/11859/; http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6712

The often-encountered problem such as protein degradation has driven various methods of cell lysis in obtaining recombinant protein post fermentation. In this paper, we compare methods such as homogenization, sonication, sonication with lysozyme and chemical lysis using B-PER reagent with lysozyme t...