Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia

This study was carried out to elucidate the different bacteria present in flood water that affected different parts of Kota Bharu, Kelantan in December, 2014 and their antibiotic sensitivity. A total of 31 water samples in 50ml sterile containers were collected from 6 locations (5 samples from each...

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Main Authors: Pwaveno Huladeino Bamaiyi, Nani Izreen, M.S, Khadijah, M, Nur Eizzati, B.H, Siti-Bainum, C.R, Norysyamimi Farhana, M.K., Mohd Norfaizul, M.N
Format: Conference or Workshop Item
Published: 2015
Online Access:http://discol.umk.edu.my/id/eprint/9115/
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spelling my.umk.eprints.91152022-05-23T11:15:55Z http://discol.umk.edu.my/id/eprint/9115/ Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia Pwaveno Huladeino Bamaiyi Nani Izreen, M.S Khadijah, M Nur Eizzati, B.H Siti-Bainum, C.R Norysyamimi Farhana, M.K. Mohd Norfaizul, M.N This study was carried out to elucidate the different bacteria present in flood water that affected different parts of Kota Bharu, Kelantan in December, 2014 and their antibiotic sensitivity. A total of 31 water samples in 50ml sterile containers were collected from 6 locations (5 samples from each of Taman Bendahara, Kampung Tok Sedang, KB clock roundabout, KB mall area, Tesco mall area and 6 samples from Jalan Kuala Krai) for bacteriological analysis by inoculating into blood agar and nutrient agar using sterile swabs. Different bacteria were identified from the cultures using colony morphology, differential/selective media and biochemical tests. Using the method of Flores et al (2009) and IDENTAX version 1.2 (2013), 38 species of bacteria were elucidated with 47% being zoonotic pathogens; 29% human pathogens; 13% Animal pathogens; 8% plant pathogens and 3% nonpathogenic bacteria. There was no significant difference (P>0.05) between the number of bacteria isolated from the different locations. Antibiotic sensitivity test indicates 78% of tested isolates were resistant to Penicillin G and Ampicillin; 67% were resistant to Amoxicillin. But, 89% of the tested isolates were susceptible to Tetracycline. This study indicate that flood waters come with a “cocktail” of bacteria that are capable of affecting humans, animals, plants and the environment. During floods human contact with the flood waters should be restricted as much as possible and prophylactic measures put in place against water and foodborne diseases that are common and rare in the tropics. Medical practitioners should consider bacterial resistance when prescribing medications during floods. 2015 Conference or Workshop Item NonPeerReviewed Pwaveno Huladeino Bamaiyi and Nani Izreen, M.S and Khadijah, M and Nur Eizzati, B.H and Siti-Bainum, C.R and Norysyamimi Farhana, M.K. and Mohd Norfaizul, M.N (2015) Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia. In: 51th Annual Scientific Conference of The Malaysian Society of Parasitology and Tropical Medicine, 03 - 04 March 2015, Grands Seasons Hotel, Kuala Lumpur.
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description This study was carried out to elucidate the different bacteria present in flood water that affected different parts of Kota Bharu, Kelantan in December, 2014 and their antibiotic sensitivity. A total of 31 water samples in 50ml sterile containers were collected from 6 locations (5 samples from each of Taman Bendahara, Kampung Tok Sedang, KB clock roundabout, KB mall area, Tesco mall area and 6 samples from Jalan Kuala Krai) for bacteriological analysis by inoculating into blood agar and nutrient agar using sterile swabs. Different bacteria were identified from the cultures using colony morphology, differential/selective media and biochemical tests. Using the method of Flores et al (2009) and IDENTAX version 1.2 (2013), 38 species of bacteria were elucidated with 47% being zoonotic pathogens; 29% human pathogens; 13% Animal pathogens; 8% plant pathogens and 3% nonpathogenic bacteria. There was no significant difference (P>0.05) between the number of bacteria isolated from the different locations. Antibiotic sensitivity test indicates 78% of tested isolates were resistant to Penicillin G and Ampicillin; 67% were resistant to Amoxicillin. But, 89% of the tested isolates were susceptible to Tetracycline. This study indicate that flood waters come with a “cocktail” of bacteria that are capable of affecting humans, animals, plants and the environment. During floods human contact with the flood waters should be restricted as much as possible and prophylactic measures put in place against water and foodborne diseases that are common and rare in the tropics. Medical practitioners should consider bacterial resistance when prescribing medications during floods.
format Conference or Workshop Item
author Pwaveno Huladeino Bamaiyi
Nani Izreen, M.S
Khadijah, M
Nur Eizzati, B.H
Siti-Bainum, C.R
Norysyamimi Farhana, M.K.
Mohd Norfaizul, M.N
spellingShingle Pwaveno Huladeino Bamaiyi
Nani Izreen, M.S
Khadijah, M
Nur Eizzati, B.H
Siti-Bainum, C.R
Norysyamimi Farhana, M.K.
Mohd Norfaizul, M.N
Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia
author_facet Pwaveno Huladeino Bamaiyi
Nani Izreen, M.S
Khadijah, M
Nur Eizzati, B.H
Siti-Bainum, C.R
Norysyamimi Farhana, M.K.
Mohd Norfaizul, M.N
author_sort Pwaveno Huladeino Bamaiyi
title Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia
title_short Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia
title_full Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia
title_fullStr Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia
title_full_unstemmed Isolation and identification of bacteria from the 2014 flood of Kota Bharu, Malaysia
title_sort isolation and identification of bacteria from the 2014 flood of kota bharu, malaysia
publishDate 2015
url http://discol.umk.edu.my/id/eprint/9115/
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score 13.211869